General atherosclerosis detection primer group for persons and machin, detection chip and detection method
An atherosclerosis and detection chip technology, which is applied in biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc. Problems such as the inconvenience of genetic diagnosis research
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0078] Example 1. Extraction of white blood cells.
[0079] Collect venous blood into anticoagulant tubes and label them separately; 7 cases in each of healthy cynomolgus monkeys, cynomolgus monkeys with atherosclerosis, healthy people, and patients with atherosclerosis. Samples 1-7 are samples of healthy cynomolgus monkeys; samples 8-14 are samples of cynomolgus monkeys with atherosclerosis; samples 15-21 are samples of healthy people; samples 22-28 are samples of patients with atherosclerosis. The atherosclerotic cynomolgus monkey mentioned above is a model monkey of atherosclerosis obtained by inducing a normal cynomolgus monkey with a high-energy diet. The relevant indicators of the obtained model monkeys were detected as follows: C-reactive protein > 10㎎ / L, cholesterol > 7.97mmol / L, low-density lipoprotein cholesterol > 6.57mmol / L, high-density lipoprotein cholesterol 1.58mmol / L, fasting blood glucose >4.62mmol / L, blood pressure ≥140 / 90mmHg. In addition, the test resul...
Embodiment 2
[0082] Example 2. Extraction of RNA.
[0083] ①Take the leukocytes obtained in Example 1 out of the liquid nitrogen, let stand at room temperature, flick the wall of the tube to disperse the leukocytes, add 1mL TRIZOL to each cryovial, and mix well.
[0084] ②Layering: After adding TRIZOL to the sample, place it at room temperature for 5 minutes to fully lyse the sample. Then add 200 μL of chloroform, vibrate vigorously and mix well, then place at room temperature for 3-5min.
[0085] ③ RNA precipitation: centrifuge at 10000g for 15min at 4°C. The sample will be divided into three layers: the yellow organic phase in the lower layer, the colorless aqueous phase in the middle and upper layers, and the RNA is mainly in the aqueous phase. Transfer the aqueous phase (usually 550 μL can be pipetted) to a new tube. Pipette the aqueous phase carefully and never pipette the intermediate interface, as this will result in DNA contamination in the RNA sample. Add an equal volume of i...
Embodiment 3
[0090] Example 3. Reverse transcription of cDNA.
[0091] Use Transgene's cDNA reverse transcription kit——EasyScript First-Strand cDNA Synthesis SuperMix (TransGen Biotech, AE301) to reverse-transcribe the RNA extracted in Example 2 into cDNA. The specific method can be carried out by referring to the instructions, and the prepared cDNA is placed in Store at -20°C until use.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com