Paraffin sectioning method for ocean shellfish D-shaped larva

A technique for paraffin sectioning and shellfish, which is applied in the preparation of test samples, sampling devices, etc., to achieve the effects of easy operation, overcoming inconvenience in operation, and simple and convenient method.

Inactive Publication Date: 2011-08-10
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional paraffin section technology has not solved many of the above difficulties, so there is no report on the use of paraffin section technology to study the development of bivalve larvae

Method used

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  • Paraffin sectioning method for ocean shellfish D-shaped larva
  • Paraffin sectioning method for ocean shellfish D-shaped larva
  • Paraffin sectioning method for ocean shellfish D-shaped larva

Examples

Experimental program
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Embodiment 1

[0037] First prepare Bouin's solution, hematoxylin staining solution and eosin staining solution.

[0038] Bouin's fixative: Take 375ml of picric acid saturated solution (prepared with 30‰ sterilized seawater), 125ml of formaldehyde and 25ml of glacial acetic acid, and mix them evenly to obtain Bouin's fixative.

[0039] Hematoxylin staining solution: hematoxylin 4g, ethanol 200ml, glacial acetic acid 20ml, glycerol 200ml, distilled water 200ml, potassium alum (KAl(SO4)2·12H2O) about 20g, sodium iodate 0.8g; Dissolve 4g in 200mL of ethanol, add 20mL of glacial acetic acid, mix, add 200mL of distilled water and 200mL of glycerin, then add potassium aluminum sulfate (about 20g) to saturation, stir well, pour it into a bottle, then add 0.8g of sodium iodate, Stir evenly until the color of the solution turns dark red to obtain the hematoxylin dye solution.

[0040] 0.1% eosin dye solution: dissolve 1g eosin in 1000ml 95% alcohol solution.

[0041] Paraffin section method of mari...

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Abstract

The invention relates to a paraffin sectioning method for an ocean shellfish D-shaped larva, which comprises the following steps: (1) Boulin solution is added to a container having an ocean shellfish D-shaped larva sample, and then alcohol with mass percentage concentration of 70% is added so as to obtain a fixed ocean shellfish D-shaped larva; (2) the fixed ocean shellfish D-shaped larva is wrapped up and is placed in a sample embedding box, and the sample embedding box is placed in a dehydrator or a staining jar; and then the steps of dehydration, transparency and wax impregnation are carried out; (3) embedding and sample location are carried out; (4) slicing and drying are carried out; and (5) a hematoxylin-eosin counterstaining method is adopted for dying. The paraffin sectioning method is simple and practical, has short processing time and transitional steps, leads all tissues of the larva to be gradually and completely immersed by reagent, well keeps the original state and structure of the tissues of the larva and provides a practical and effective technical means for research on early stage embryos and larva development of shellfishes.

Description

technical field [0001] The invention belongs to the field of paraffin sectioning of larvae, in particular to a method for paraffin sectioning of marine shellfish D-shaped larvae. Background technique [0002] Paraffin section technology is one of the important research methods of histology and embryology. Its technical means are mature and stable, and it has the characteristics of complete structure of tissue embryos, clear images obtained, and strong contrast. Tissue embryos can be clearly seen under an ordinary optical microscope. The external morphology and internal structure have unique advantages that are different from compression, frozen section and electron microscopy. Paraffin section technique has been widely used in histology, embryology and cytology of fish and mammalian embryos and larvae. [0003] Since most marine shellfish start from D-shaped larvae, the surface of the larvae begins to cover a layer of calcareous larval shell, which cannot be peeled off by h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/06G01N1/30
Inventor 李子牛周凯来琦芳么宗利应成琦
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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