Paraffin sectioning method for ocean shellfish D-shaped larva

A technique for paraffin sectioning and shellfish, which is applied in the preparation of test samples, sampling devices, etc., to achieve the effects of easy operation, overcoming inconvenience in operation, and simple and convenient method.
CN102147337AInactive Publication Date: 2011-08-10EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2011-08-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a paraffin sectioning method for an ocean shellfish D-shaped larva, which comprises the following steps: (1) Boulin solution is added to a container having an ocean shellfish D-shaped larva sample, and then alcohol with mass percentage concentration of 70% is added so as to obtain a fixed ocean shellfish D-shaped larva; (2) the fixed ocean shellfish D-shaped larva is wrapped up and is placed in a sample embedding box, and the sample embedding box is placed in a dehydrator or a staining jar; and then the steps of dehydration, transparency and wax impregnation are carried out; (3) embedding and sample location are carried out; (4) slicing and drying are carried out; and (5) a hematoxylin-eosin counterstaining method is adopted for dying. The paraffin sectioning method is simple and practical, has short processing time and transitional steps, leads all tissues of the larva to be gradually and completely immersed by reagent, well keeps the original state and structure of the tissues of the larva and provides a practical and effective technical means for research on early stage embryos and larva development of shellfishes.
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Description

technical field

[0001] The invention belongs to the field of paraffin sectioning of larvae, in particular to a method for paraffin sectioning of marine shellfish D-shaped larvae. Background technique

[0002] Paraffin section technology is one of the important research methods of histology and embryology. Its technical means are mature and stable, and it has the characteristics of complete structure of tissue embryos, clear images obtained, and strong contrast. Tissue embryos can be clearly seen under an ordinary optical microscope. The external morphology and internal structure have unique advantages that are different from compression, frozen section and electron microscopy. Paraffin section technique has been widely used in histology, embryology and cytology of fish and mammalian embryos and larvae.

[0003] Since most marine shellfish start from D-shaped larvae, the surface of the larvae begins to cover a layer of calcareous larval shell, which cannot be peeled off by h...

Claims

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