Method for identifying salt resisting function of gene

A functional and gene silencing technology, applied in the biological field, can solve the problem of lack of salt stress-related genes in plants, limited and other problems, and achieve the effect of accurate identification results.

Inactive Publication Date: 2011-08-17
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

At present, unlike the research in biotic stress, the application of VIGS in the study of abiotic stress is sti...

Method used

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  • Method for identifying salt resisting function of gene
  • Method for identifying salt resisting function of gene
  • Method for identifying salt resisting function of gene

Examples

Experimental program
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Embodiment 1

[0042] Example 1. Silencing and detection of plant endogenous LeNHX2 gene related to salt resistance

[0043] 1. Identifying that the expression of LeNHX2 gene is regulated by NaCl

[0044] Take the 30-day-old tomato variety Lichun, and treat it with 200mM NaCl for 0, 3, 6, 12, 24, and 48 hours, then take the upper mature and fully expanded leaves of the tomato variety Lichun treated with NaCl at different times, and extract total RNA. , reverse-transcribed into cDNA, Actin was used as an internal reference, and LeNHX2-specific primers were used for Real-timequantitative PCR analysis, the results were as follows figure 1 shown. From figure 1 It can be seen that, compared with 0 hour without NaCl treatment, the expression of LeNHX2 gene increased under NaCl treatment and reached the highest at 6 hours. This result indicated that the LeNHX2 gene was involved in the response of plants to salt stress.

[0045] 2. Identification of the function of LeNHX2 gene by virus-induced g...

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Abstract

The invention discloses a method for identifying the salt resisting function of a gene. The method for detecting whether the target gene in a plant has the salt resisting function provided by the invention comprises the following steps: losing the functions of the target gene in the target plant by use of a virus-induced gene silencing method, and marking the obtained plant as a gene silencing plant; performing salt stress processing on the gene silencing plant; detecting the salt-stress-related character of the gene silencing plant after the salt stress processing; and if the gene silencing plant after the salt stress processing shows the salt resisting character, determining that the target gene has the salt resisting function. Experiments demonstrate that: the method disclosed by the invention offers correct identification results, and has important significance on quickly identifying and exploring the salt-resistance-related gene resources.

Description

technical field [0001] The invention relates to a method for identifying the salt resistance function of a gene in the field of biotechnology. Background technique [0002] Soil salinization is a global problem affecting agricultural production and ecological environment. Salinity is one of the two major soil factors restricting agricultural production in my country. The salinity in the soil is an important factor affecting the growth of plants. Soil salinization seriously hinders the growth and development of plants, resulting in the reduction of crop yield. At present, about 20% of the world's agricultural land is threatened by salinization, and it is estimated that by 2050, more than 50% of the world's land will become salinized. In the 1940s, scientists from various countries began to study the salt tolerance of plants. Especially in recent years, both the screening of salt-tolerant varieties and the research on the mechanism of salt-tolerant varieties have made great p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/82
Inventor 李银心贺希格都楞陈显扬
Owner INST OF BOTANY CHINESE ACAD OF SCI
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