Cell recognition and detection method based on biochemical reagent of supermolecule nanometer self-assembled system

A nano-self-assembly and detection method technology, applied in the field of tumor cell detection, can solve problems such as limited application and difficult dispersion, and achieve the effects of improving detection sensitivity, facilitating operation, and simple operation

Inactive Publication Date: 2011-08-17
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since CNTs are almost insoluble in any solvent and tend to aggregate into bundles in the solution, it is d

Method used

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  • Cell recognition and detection method based on biochemical reagent of supermolecule nanometer self-assembled system
  • Cell recognition and detection method based on biochemical reagent of supermolecule nanometer self-assembled system
  • Cell recognition and detection method based on biochemical reagent of supermolecule nanometer self-assembled system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of a nano-interface-based biosensor

[0030] Ultrasonic dispersion of 10mg β-cyclodextrin (b-CD) and 1mg carbon nanotubes in 1mL dimethylformamide (DMF) gave a black suspension; the glassy carbon electrode was coated with 0.05 μm aluminum powder on suede Polish to a mirror surface, wash in acetone, absolute ethanol, and ultrapure water, and blow dry. Take 3 μL of the prepared composite suspension and evenly drop-coat it on the surface of the treated glassy carbon electrode, and place it in a desiccator until the solvent evaporates to obtain a nanocomposite modified electrode (β-CD / CNT / GCE).

[0031] Carbon nanotubes and β-cyclodextrin / carbon nanotube composites were characterized by TEM. Carbon nanotubes are usually entangled randomly and distributed in agglomerates in the solution, but after adding β-cyclodextrin, the distribution of carbon nanotubes is relatively dispersed, and the original intertwined agglomerates are untied, which is conducive...

Embodiment 2

[0032] Example 2 Preparation of a nano-interface-based biosensor

[0033]Ultrasonic disperse 10mg of β-cyclodextrin (b-CD) and 1mg of carbon nanotubes in 1mL of dimethylformamide (DMF) to obtain a black suspension; use ITO electrodes, carbon paper or carbon cloth and other electrodes as required The required specifications were cut, and 3 μL of the prepared composite suspension was evenly dripped on the surface of the relevant electrode, and placed in a desiccator until the solvent evaporated to obtain the nanocomposite modified electrode.

Embodiment 3

[0034] Example 3 Preparation of a nano-interface-based biosensor

[0035] Ultrasonic dispersion of 0.5mg graphene or graphene composite nanomaterials in 1ml chitosan solution (0.5mg / ml, dissolved in 1% acetic acid solution, potassium hydroxide to adjust PH=5) to obtain a uniformly dispersed suspension ; The glassy carbon electrode was polished to a mirror surface on the suede with 0.05 μm aluminum powder, washed in acetone, absolute ethanol, and ultrapure water, and dried. Take 2 μL of the prepared graphene or graphene composite nanomaterial suspension and evenly drop-coat it on the surface of the treated glassy carbon electrode, and place it in a desiccator until the solvent evaporates to obtain a nano-modified electrode.

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Abstract

The invention discloses a cell recognition and detection method based on a biochemical reagent of a supermolecule nanometer self-assembled system. The method comprises the following steps of: step one. a carbon nanotube or a graphite composite nanometer material is ultrasonically dispersed in a corresponding solvent so as to obtain a suspension liquid; step two. the suspension liquid prepared in the step one is uniformly and dropwise coated on the surface of an electrode, and the electrode is put in a dryer till a solvent volatilizes so as to obtain a nanometer self-assembled system modified electrode; step three. normal cells or tumor cells are washed and diluted with phosphate buffer, and then are uniformly coated on the surface of the nanometer modified electrode prepared in the step 2, the nanometer modified electrode is placed in an incubator at a constant temperature of 37 DEG C for 2h so as to obtain a cell sensor based on a nanometer interface; finally, by the using of the sensor, electroactive biochemical reagents such as tetrathiafulvalene derivatives or carborane derivatives and the like are used as probe molecules, and methods such as electrochemistry and surface contact angle detection and the like are used for carrying out high sensitivity detection and analysis on different types of cells.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis and detection, in particular to a tumor cell detection method. Background technique [0002] In the effective treatment of cancer, timely and early diagnosis of cancerous tissues and cells is one of the most effective methods. However, in reality, due to the lack of obvious early symptoms of cancer, it is difficult to be detected in time, and the current conventional histopathological diagnosis methods are difficult to detect the biochemical and molecular abnormalities in diseased tissues and cells in the early stage. Therefore, it is of great significance to find a convenient, fast and accurate method for early diagnosis of cancer. Researching more advanced means or instruments to achieve accurate and timely early detection of cancer has always been one of the directions of people's efforts. [0003] Biosensing technology is a high-tech that has grown through the interpenetration of...

Claims

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Application Information

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IPC IPC(8): B82Y5/00C12Q1/02
Inventor 王雪梅吴晓静赵娟常虎成张海军左景林
Owner SOUTHEAST UNIV
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