New method for identifying and evaluating bulbus fritillariae cirrhosae
A technique of fritillary fritillary and a new method, applied in the field of identification and evaluation of the traditional Chinese medicinal material fritillary fritillary, can solve the problems such as not being able to fully distinguish
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[0032] Use dark purple Fritillaria from Xining and Maqin in Qinghai, Gansu Fritillaria from Guide, Qinghai Xining, and Luhuo in Ganzi Prefecture, Curly-leaf Fritillaria from Kangding County, Sichuan, and Susia Fritillaria from Huding County, Sichuan Each of the 6 samples was used as the object, and about 1.2g of the crushed fritillary mother sample (passed through a 80-mesh sieve) was weighed, and 15ml of methanol was added, weighed, refluxed in a water bath at 55°C for 90min; weighed again, and made up to before the water bath weight, filtered, discarded the initial filtrate, took 10ml of the continued filtrate, concentrated to dryness under reduced pressure, and dried in vacuum for 30 minutes; the residue was washed with 0.05ml DMSO-d 6 Dissolved, transferred to f5mm nuclear magnetic tube, that is available for 1 Sample solution for H-NMR spectrum determination.
[0033] At constant temperature at 296K, with DMSO-d 6 As an internal lock, scan 128 times, the sampling time d...
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