Method for preparing pig-bile-combination-type total bile acid and application thereof

A combined type, pig bile technology, applied in the treatment of colonic inflammation, the field of preparation of combined total bile acid, to achieve the effect of blocking damage

Active Publication Date: 2011-09-14
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of porcine bile-bound total bile acid in pharmaceuticals has not been reported yet

Method used

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  • Method for preparing pig-bile-combination-type total bile acid and application thereof
  • Method for preparing pig-bile-combination-type total bile acid and application thereof
  • Method for preparing pig-bile-combination-type total bile acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Taking fresh pig bile method 100mL as an example, the preparation method of pig bile-bound cholic acid is as follows:

[0024] 1. Ethanol extraction

[0025] Take fresh pig bile, add 500mL of ethanol with a mass fraction of 95%, let it stand for 12-14 hours, filter, recover the solvent, and obtain a brown-black extract;

[0026] 2. Separation and purification

[0027] Take 2g of the above-mentioned extract, add 4g of 100-160 mesh column chromatography silica gel to mix the sample, evaporate the solvent, and grind into 80 mesh fine powder; dry the obtained fine powder and neutral alumina according to the mass ratio of 1:15 In the chromatographic column, elute with ethanol with a mass fraction of 95%, collect the eluate, and spot the sample on a silica gel G thin-layer plate with a capillary tube. The volume ratio of ethyl acetate to methanol, water, and glacial acetic acid is 10 : 2: 1: 0.1 mixed developing agent, developed with vanillin sulfuric acid test solution col...

Embodiment 2

[0031] Taking 100 mL of fresh pig bile as an example, the preparation method of pig bile-bound cholic acid is as follows:

[0032] In the separation and purification step 2, take 2 g of the above extract, add 4 g of 100-160 mesh column chromatography silica gel to mix the sample, evaporate the solvent, and grind into 80 mesh fine powder; the obtained fine powder and neutral alumina are mixed according to the mass ratio of 1 The ratio of : 10 is installed in the chromatographic column, and the ethanol with a mass fraction of 95% is used for eluting, and the eluate is collected, and the sample is spotted on a silica gel G thin-layer plate with a capillary tube, and ethyl acetate, methanol, water, The volume ratio of glacial acetic acid is 10:2:1:0.1 and the mixed developing agent is developed, and the color is developed with the vanillin sulfuric acid test solution color reagent, and the eluents are combined when the spots of conjugated hyodeoxycholic acid appear on the TLC plate...

Embodiment 3

[0035] Taking 100 mL of fresh pig bile as an example, the preparation method of pig bile-bound cholic acid is as follows:

[0036] In the separation and purification step 2, take 2 g of the above extract, add 4 g of 100-160 mesh column chromatography silica gel to mix the sample, evaporate the solvent, and grind into 80 mesh fine powder; the obtained fine powder and neutral alumina are mixed according to the mass ratio of 1 The ratio dry method of : 20 is installed in the chromatographic column, eluting with 95% ethanol with mass fraction, collecting the eluate, spotting the sample on the silica gel G thin-layer plate with a capillary, and using ethyl acetate, methanol, water, The volume ratio of glacial acetic acid is 10:2:1:0.1 and the mixed developing agent is developed, the color is developed with the vanillin sulfuric acid test solution, and the eluents are combined when the spot of conjugated hyodeoxycholic acid appears on the TLC plate , until the spots disappear.

[0...

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Abstract

The invention relates to a method for preparing pig-bile-combination-type total bile acid. The method comprises the steps of extracting by utilizing ethanol, separating and purifying and drying. Proved by medicinal effectiveness tests for treating colonitis, the pig-bile-combination-type total bile acid prepared by utilizing the method provided by the invention has an inhibitory action on the increase of myeloperoxidase activity and tumor necrosis factor alpha content of model mice and has the advantages of inhibiting the secretion of proinflammatory cytokine, regulating the immune response, effectively blocking the damage of the immunoreactivity to an organism and achieving the purpose of treating colonic inflammation. The pig-bile-combination-type total bile acid prepared by utilizing the method provided by the invention can be adopted as a raw material to be made into various commonly-used preparation types such as tablets, capsules, powders, pills, granules, enemas and the like.

Description

technical field [0001] The invention relates to the application of porcine bile-bound total cholic acid in medicaments, in particular to the preparation of the combined total cholic acid and its application in treating colon inflammation. Background technique [0002] Ulcerative colitis, also known as chronic nonspecific colitis, is a chronic idiopathic inflammatory reaction that mainly involves the colon and rectum, and is a type of inflammatory bowel disease. Many studies have confirmed that colitis is mainly manifested by the serious imbalance of the interaction between the autoimmune system and internal environmental factors. It is a chronic inflammation and ulcerative lesion of the large intestine mucosa with unknown causes. The pathological changes are superficial, diffuse, continuous, etc. features. Colitis lesions are mainly limited to the large intestinal mucosa and submucosa, clinically characterized by diarrhea, mucous pus and blood in the stool, and abdominal pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413A61P1/00A61P1/04
Inventor 何姣孙文基谢人明
Owner NORTHWEST UNIV(CN)
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