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SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon

A technique for gsb-4 and blight, which is applied in the detection/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of long cycle, low efficiency, and inability to provide disease resistance, etc., to speed up the process , detection convenient and fast effect

Inactive Publication Date: 2011-09-28
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to study and screen out the resistance to vine blight of muskmelon blight for the defect that the resistance gene Gsb-1 of melon vine blight cannot provide sufficient and durable disease resistance, and the problems of low efficiency and long cycle of conventional breeding improvement methods. Molecular marker of the sex gene Gsb-4, providing genetic resources and marker-assisted selection technology for the breeding of muskmelon blight resistance

Method used

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  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon
  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon
  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon

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Embodiment 1

[0031] The implementation procedure of the molecular marker of the muskmelon blight resistance gene Gsb-4 of the present invention comprises:

[0032] a) The disease-resistant inbred line PI482398 ( F 1 , F 1 F 2 , while backcrossing with the parents respectively to produce BC 1 f 1 and BC 2 f 1 Separate groups. In the autumn of 2009, he was awarded F in the experimental base of the School of Horticulture, Nanjing Agricultural University 2 、BC 1 f 1 and BC 2 f 1 generational segregation groups. Spring 2010 vs BC 1 f 1 、BC 2 f 1 Separate groups and F 2 The isolated populations were artificially inoculated and the disease incidence of each individual plant was counted, and the genetic law of the resistance to the blight was analyzed by chi-square test. Analysis found 108 strains of BC 1 f 1 Except for the 2 strains identified in the inoculation as susceptible, the rest were resistant in the inoculation, and 109 strains of BC 2 f 1 The resistant / susceptible...

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Abstract

The invention belongs to the technical field of crop genetic and breeding and biology and relates to an SSR (Simple Sequence Repeats) marker of a gummy stem blight resistant gene Gsb-4 of a melon resource PI482398; an SSR primer marker CMAT170a is utilized to amplify a breeding material or a variety DNA of the gummy stem blight resistant gene Gsb-4 of the melon resource PI482398 to obtain a 120 bp amplified fragment which is the SSR marker of the gummy stem blight resistant gene Gsb-4 of the melon resource PI482398; and the genetic distance from the SSR marker to a resistant gene is 5.14cM. In the invention, an SSR molecular marker tightly linked with the gummy stem blight resistant gene Gsb-4 of the melon is internationally obtained for the first time. The gummy stem blight resistance of the melon can be predicted by detecting the molecular marker and further a disease-resistant variety or a strain is quickly screened out for breeding the melons. The disease-resistant gene locus is convenient and quick to detect without being limited by environmental conditions.

Description

technical field [0001] The invention belongs to the field of crop genetic breeding and biotechnology, and relates to the SSR marker of the muskmelon blight resistance gene Gsb-4. technical background [0002] Melon is one of the top ten fruits in the world. Blight is one of the serious global diseases of melon. Its occurrence often leads to devastating consequences. The control of muskmelon blight has become an urgent task in the current muskmelon production. Breeding disease-resistant varieties is one of the safest and most effective measures to control the damage of muskmelon blight. [0003] The traditional breeding method is through inoculation identification and screening according to the phenotypic characteristics of the plants. This method sometimes affects the identification effect due to insufficient inoculation or unsuitable disease conditions, and it is difficult to accurately and quickly screen out individual plants with disease-resistant genes. Molecular mar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈劲枫王红英钱春桃郭勤卫薄凯亮
Owner NANJING AGRICULTURAL UNIVERSITY
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