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SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon

A technology of gsb-4, vine blight, applied in DNA/RNA fragments, recombinant DNA technology and other directions, can solve the problems of long cycle, low efficiency, inability to provide disease resistance, etc., to achieve the effect of convenient and rapid detection and accelerated process

Inactive Publication Date: 2012-10-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to study and screen out the resistance to vine blight of muskmelon blight for the defect that the resistance gene Gsb-1 of melon vine blight cannot provide sufficient and durable disease resistance, and the problems of low efficiency and long cycle of conventional breeding improvement methods. Molecular marker of the sex gene Gsb-4, providing genetic resources and marker-assisted selection technology for the breeding of muskmelon blight resistance

Method used

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  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon
  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon
  • SSR (Simple Sequence Repeats) marker of gummy stem blight resistant gene Gsb-4 of melon

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Embodiment 1

[0031] The implementation procedures for the molecular marker of the muskmelon resistance gene Gsb-4 of the present invention include:

[0032] a) The disease-resistant inbred line PI482398 ( Introduced from the United States) and ‘white skin crisp’ (♀, purchased from Xinjiang Academy of Agricultural Sciences) to obtain F 1 , F 1 Selfing produces F 2 , And at the same time backcross with parents to produce BC 1 F 1 And BC 2 F 1 Separated groups. Obtained F in the experimental base of College of Horticulture, Nanjing Agricultural University in the fall of 2009 2 , BC 1 F 1 And BC 2 F 1 Generation segregated groups. BC in the spring of 2010 1 F 1 , BC 2 F 1 Separated group and F 2 The isolated populations were inoculated artificially and the incidence of each individual plant was counted. Chi-square detection was used to analyze the genetic law of vine blight resistance. Analysis found 108 strains of BC 1 F 1 Except for the 2 strains identified in the inoculation, the others showe...

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Abstract

The invention belongs to the technical field of crop genetic and breeding and biology and relates to an SSR (Simple Sequence Repeats) marker of a gummy stem blight resistant gene Gsb-4 of a melon resource PI482398; an SSR primer marker CMAT170a is utilized to amplify a breeding material or a variety DNA of the gummy stem blight resistant gene Gsb-4 of the melon resource PI482398 to obtain a 120 bp amplified fragment which is the SSR marker of the gummy stem blight resistant gene Gsb-4 of the melon resource PI482398; and the genetic distance from the SSR marker to a resistant gene is 5.14cM. In the invention, an SSR molecular marker tightly linked with the gummy stem blight resistant gene Gsb-4 of the melon is internationally obtained for the first time. The gummy stem blight resistance ofthe melon can be predicted by detecting the molecular marker and further a disease-resistant variety or a strain is quickly screened out for breeding the melons. The disease-resistant gene locus is convenient and quick to detect without being limited by environmental conditions.

Description

Technical field [0001] The invention belongs to the field of crop genetic breeding and biotechnology, and relates to the SSR marker of the muskmelon resistance gene Gsb-4. technical background [0002] Melon is one of the top ten fruits in the world. Vine blight is one of the serious global diseases of melon. Its occurrence often leads to devastating consequences. The prevention and control of muskmelon vine blight has become an urgent task in current melon production. Cultivating disease-resistant varieties is one of the safest and effective measures to prevent and control the harm of melon vine blight. [0003] The traditional breeding method is through inoculation identification and screening according to the phenotypic characteristics of the plants. This method sometimes affects the identification effect due to insufficient inoculation or unsuitable disease conditions, and it is difficult to accurately and quickly screen individual plants with disease resistance genes. Mole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11
Inventor 陈劲枫王红英钱春桃郭勤卫薄凯亮
Owner NANJING AGRICULTURAL UNIVERSITY
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