Preparation process for extracting crude sodium heparin from pig lungs

A preparation process and heparin sodium technology are applied in the field of preparation technology for extracting crude heparin sodium by utilizing pig lungs, which can solve the problems of inability to meet a large number of demands, long production cycle, difficulty in large-scale production, etc., and achieve improved and stable quality and heparin Effects of sodium yield, low production cost, and easy availability of sources

Inactive Publication Date: 2011-10-12
天津宝迪农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in my country, pig small intestine is usually used to extract fresh intestinal mucosal fluid, and the traditional salt solution process is used to produce heparin sodium. Due to the long production cycle

Method used

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Examples

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Example Embodiment

[0020] The present invention will be described in further detail below through specific examples. The following examples are only descriptive and not restrictive, and cannot limit the protection scope of the present invention.

[0021] The special enzymes for heparin sodium used in this embodiment are all the special enzymes for heparin sodium extraction produced by Nanning Donghenghuadao Biotechnology Co., Ltd.

[0022] A preparation process for extracting crude heparin sodium from pig lungs, the steps are as follows:

[0023] (1) Pulp breaking: First, clean fresh pig lungs carefully with clean water to remove internal and external dirt and external cortical fat, then mince them and grind them into a slurry. Take 1000g of pig lung slurry and add it under full stirring. 1000g water is mixed to obtain pig lung serum;

[0024] (2) Enzymatic hydrolysis: Under fully stirring the pig lung slurry, adjust the pH of the slurry with sodium hydroxide solution to 8.5, add 10g heparin sodium spec...

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Abstract

The invention relates to a preparation process for extracting crude sodium heparin from pig lungs, which comprises the following steps: mincing fresh pig lungs into slurry, carrying out heat insulation and enzymolysis to obtain an enzymolysis liquid, filtering the enzymolysis liquid to collect filtrate, carrying out ion exchange adsorption treatment on the filtrate, washing and eluting resin, precipitating sodium heparin, and drying to obtain crude sodium heparin. The enzymolysis method employed by the invention can thoroughly dissolve pig lung slurry and effectively improve and stabilize the quality of the product and the yield of sodium heparin; and simultaneously, the obtained crude sodium heparin has low impurity content and high titer.

Description

technical field [0001] The invention relates to a preparation process for extracting heparin sodium from animal organs, in particular to a preparation process for extracting crude heparin sodium from pig lungs. Background technique [0002] Heparin sodium is a glucosaminoglycan widely present in animal organs (such as small intestinal mucosa, liver, pancreas, and lung tissue of cattle and sheep), and exists as a protein-bound complex in the body like most mucopolysaccharides , the anticoagulant activity of this complex increases with the removal of the protein. Heparin sodium and its derivatives are classic anticoagulant and antithrombotic drugs, which are widely used clinically to treat hemorrhage in patients with kidney disease, acute myocardial infarction, prevent thrombosis, cerebrovascular disease, skin disease, and eliminate the formation of kidney disease Heparin sodium also has a good effect in reducing blood fat and immunity, and with the gradual in-depth understan...

Claims

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Application Information

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IPC IPC(8): C08B37/10C12S3/02
Inventor 臧盛于福满程榆茗姜无边
Owner 天津宝迪农业科技股份有限公司
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