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Preparation process for extracting crude sodium heparin from pig lungs

A preparation process, the technology of heparin sodium, is applied in the field of preparation process of crude heparin sodium extracted from pig lungs, which can solve the problems of not being able to meet a large amount of demand, difficulty in large-scale production, low purity of heparin sodium, etc., and achieve improvement and stability of heparin sodium The effect of yield, easy source and high potency

Inactive Publication Date: 2012-11-28
天津宝迪农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in my country, pig small intestine is usually used to extract fresh intestinal mucosal fluid, and the traditional salt solution process is used to produce heparin sodium. Due to the long production cycle and incomplete hydrolysis of this preparation method, the obtained heparin sodium has low purity and low yield. Low, low potency, difficult to carry out large-scale production, and cannot meet the large demand of the market

Method used

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Examples

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Embodiment Construction

[0020] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0021] The special enzymes for heparin sodium used in this example are the special enzymes for extracting heparin sodium produced by Nanning Dongheng Huadao Biotechnology Co., Ltd.

[0022] A preparation process for extracting heparin sodium crude product from pig lungs, the steps are as follows:

[0023] (1) Pulp breaking: First, wash the fresh pig lung carefully with clean water to remove the internal and external dirt and external cortical fat, then mince it and grind it into a slurry, take 1000g of the pig lung slurry, add it under full stirring 1000g of water was mixed to obtain pig lung slurry;

[0024] (2) Enzymatic hydrolysis: under sufficient stirring, adjust the pH value of the slurry to 8.5 with sodium hydroxide solution, add 10 ...

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Abstract

The invention relates to a preparation process for extracting crude sodium heparin from pig lungs, which comprises the following steps: mincing fresh pig lungs into slurry, carrying out heat insulation and enzymolysis to obtain an enzymolysis liquid, filtering the enzymolysis liquid to collect filtrate, carrying out ion exchange adsorption treatment on the filtrate, washing and eluting resin, precipitating sodium heparin, and drying to obtain crude sodium heparin. The enzymolysis method employed by the invention can thoroughly dissolve pig lung slurry and effectively improve and stabilize thequality of the product and the yield of sodium heparin; and simultaneously, the obtained crude sodium heparin has low impurity content and high titer.

Description

technical field [0001] The invention relates to a preparation process for extracting heparin sodium from animal organs, in particular to a preparation process for extracting crude heparin sodium from pig lungs. Background technique [0002] Heparin sodium is a glucosaminoglycan widely present in animal organs (such as small intestinal mucosa, liver, pancreas, and lung tissue of cattle and sheep), and exists as a protein-bound complex in the body like most mucopolysaccharides , the anticoagulant activity of this complex increases with the removal of the protein. Heparin sodium and its derivatives are classic anticoagulant and antithrombotic drugs, which are widely used clinically to treat hemorrhage in patients with kidney disease, acute myocardial infarction, prevent thrombosis, cerebrovascular disease, skin disease, and eliminate the formation of kidney disease Heparin sodium also has a good effect in reducing blood fat and immunity, and with the gradual in-depth understan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10C12S3/02
Inventor 臧盛于福满程榆茗姜无边
Owner 天津宝迪农业科技股份有限公司