Collagen temperature-sensitive hydrogel and preparation method thereof

A collagen and temperature-sensitive technology, applied in medical science, prosthesis, etc., can solve the problems of allergic degradation, redness and swelling of the use site, too fast, etc., to achieve weak immune rejection, long degradation time, safe and effective application. Effect

Active Publication Date: 2012-11-28
XIAN GIANT BIOGENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, F Dalton of the United States has successively approved several products for soft tissue filling, the main components of which are bovine collagen, sodium hyaluronate, PMMA pellets, etc. Patients still have redness and swelling at the site of use, allergies and rapid degradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Step 1: dissolving animal-derived collagen with water for injection into a solution with a mass fraction of 1.0%;

[0039] Step 2: the degree of deacetylation is 80%, the molecular weight is 10 5 Dalton's chitosan is dissolved into a solution of 0.5% by mass fraction in dilute hydrochloric acid of 0.5%;

[0040] Step 3: dissolving β-sodium glycerophosphate with water for injection into a solution with a mass fraction of 20%;

[0041] Step 4: Mix the above three solutions at a volume ratio of 1:1:1, then stir at 0°C for 10 minutes to mix evenly, and adjust the pH to 7.4 at the same time;

[0042] Step 5: Fill the above mixture under aseptic conditions.

[0043] This example hydrogel material is through following test:

[0044] Transfer the prepared mixture into a 1mL syringe and store at 4°C for later use. Select 50 excellent Kunming mice, male, weighing 25-30g, 25 as the experimental group, and 25 as the negative control group. They were fed routinely one week befor...

Embodiment 2

[0051] Step 1: dissolving animal-derived collagen with water for injection into a solution with a mass fraction of 2.5%;

[0052] Step 2: The degree of deacetylation is 85%, the molecular weight is 2.5×10 5 Dalton's chitosan is dissolved into a solution of 1.2% by mass fraction in dilute acetic acid of 0.5%;

[0053] Step 3: dissolving β-sodium glycerophosphate with water for injection into a solution with a mass fraction of 35%;

[0054] Step 4: Mix the above three solutions at a volume ratio of 2:2:1, stir at 5°C for 15 minutes, mix well, and adjust the pH to 7.4 at the same time;

[0055] Step 5: Fill the above mixture under aseptic conditions.

[0056] This example hydrogel material is through following test:

[0057] Transfer the prepared mixture into a 1mL syringe and store at 4°C for later use. Select 50 excellent Kunming mice, male, weighing 25-30g, 25 as the experimental group, and 25 as the negative control group. They were fed routinely one week before the test....

Embodiment 3

[0064] Step 1: dissolving animal-derived collagen with water for injection into a solution with a mass fraction of 5.0%;

[0065] Step 2: The degree of deacetylation is 85%, the molecular weight is 5×10 5 Dalton's chitosan is dissolved into a solution of 2.0% by mass fraction with 1% dilute lactic acid;

[0066] Step 3: dissolving β-sodium glycerophosphate with water for injection into a solution with a mass fraction of 50%;

[0067] Step 4: Mix the above three solutions at a volume ratio of 3:3:1, then stir at 5°C for 20 minutes to mix evenly, and adjust the pH to 7.4 at the same time;

[0068] Step 5: Fill the above mixture under aseptic conditions.

[0069] This example hydrogel material is through following test:

[0070] Transfer the prepared mixture into a 1mL syringe and store at 4°C for later use. Select 50 excellent Kunming mice, male, weighing 25-30g, 25 as the experimental group, and 25 as the negative control group. They were fed routinely one week before the t...

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Abstract

The invention relates to a collagen temperature-sensitive hydrogel and a preparation method thereof Soft tissue filling materials utilized in the existing plastic and beauty treatment industry have the problems of hardening, production of phyma, too fast absorption speed, and induction of chronic inflammation. American F Dalton approves successively multiple products for soft tissue filling, and main components of the products comprise bovine collagen, hyaluronic acid sodium, polymethyl methacrylate (PMMA) globules and the like. The products have good effects but can still produce cases of redness and allergy of a used position and too fast absorption. In the invention, collagen solution, chitosan acid or hyaluronic acid, and beta-sodium glycerophosphate solution are mixed according to a volume ratio of (1 to 3): (1 to 5): (1 to 2), then are stirred well at a temperature of 0 to 15 DEG C for 10 to 30 minutes and are adjusted to a pH of 7.4 to form temperature-sensitive hydrogel. The temperature-sensitive hydrogel has the advantages of good gel forming property at a temperature of 37 DEG C, gel forming time within 10 minutes, good mechanical strength, long degradation time, low immunological rejection, and favorable safety and effectiveness.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a thermosensitive collagen hydrogel and a preparation method thereof. Background technique [0002] In the plastic surgery industry, soft tissue filling materials are needed for removing wrinkles, improving facial contours, filling sunken scars or lips, and repairing injured tissues. People have successively studied white wax, fat grafting, silicone resin, gel implants and other materials. For soft tissue plastic surgery, it is found in clinical application that there are problems such as sclerosis, tumor-like mass, rapid absorption, and chronic inflammation. In recent years, F Dalton of the United States has successively approved several products for soft tissue filling, the main components of which are bovine collagen, sodium hyaluronate, PMMA pellets, etc. Patients still have redness and swelling at the site of use, allergies, and rapid degradation. Contents ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00C08L5/08A61L27/24A61L27/20A61L27/52
Inventor 范代娣段志广马晓轩严建亚
Owner XIAN GIANT BIOGENE TECH CO LTD
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