High-flux fluorescent McAb nanometer microsphere kit

A nano-microsphere, fluorescent nano-technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome steps, difficult to standardize, and unable to be used as a routine inspection for diagnostic ITP.

Inactive Publication Date: 2011-11-02
苏州博赛生物医药有限公司 +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

In 1995, Koksch et al. used flow cytometry fluorescence resonance energy transfer to measure autoantibodies against specific sites on platelet memb

Method used

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Embodiment Construction

[0024] The invention provides a high-throughput fluorescent monoclonal antibody nano-microsphere kit, and at the same time provides a method for detecting platelet-specific autoantibodies using the high-throughput fluorescent monoclonal antibody nano-microsphere kit, and also provides a high-throughput fluorescent monoclonal antibody The preparation method of the main components in the anti-nanosphere kit will be further described below through specific examples.

[0025] First, the main components in the high-throughput fluorescent monoclonal antibody nanosphere kit are prepared by the following steps:

[0026] Step A, prepare PBS liquid (NaCl0.15mol / L, NaCl 2 HPO 4 0.01mol / L and NaH 2 PO 4 0.01mol / L mixed solution, pH7.4), as the component g in the high-throughput fluorescent monoclonal antibody nanosphere kit;

[0027] Step B. Dilute monoclonal antibodies SZ1, SZ2, SZ21, SZ22, and SZ51 with carbonate buffer solution to about 16 μg each, and add fluorescent nanospheres 1...

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Abstract

The present invention aims to provide a high-flux fluorescent McAb nanometer microsphere kit which can rapidly, easily and accurately detect platelet antoantibodies so as to make an early diagnosis of autoimmunity diseases, comprising a, a first fluorescent nanometer microsphere solution blocked by the bovine serum albumin and connected with the monoclonal antibody SZ-1; b, a second fluorescent nanometer microsphere solution blocked by the bovine serum albumin and connected with the monoclonal antibody SZ-2; c, a third fluorescent nanometer microsphere solution blocked by the bovine serum albumin and connected with the monoclonal antibody SZ-21; d, a fourth fluorescent nanometer microsphere solution blocked by the bovine serum albumin and connected with the monoclonal antibody SZ-22; e, a fifth fluorescent nanometer microsphere solution blocked by the bovine serum albumin and connected with the monoclonal antibody SZ-51; f, fluorescein isothiocyanate connected goat anti-human polyclonal antibodies; g, a PBS liquid. The invention also provides an application of the kit and a preparation method of main components of the kit.

Description

technical field [0001] The invention relates to the field of diagnostic kits, in particular to a high-throughput fluorescent monoclonal antibody nano-microsphere kit. Background technique [0002] Idiopathic thrombocytopenic purpura (ITP) is an immune-mediated thrombocytopenia syndrome. It is the most common bleeding disorder in clinical practice, accounting for about 30% of the total number of bleeding disorders. It is generally believed that the disease is caused by excessive destruction of platelets sensitized by autoantibodies by the mononuclear macrophage system. It is characterized by spontaneous bleeding, thrombocytopenia, prolonged bleeding time and poor blood clot contraction, and the development of megakaryocytes in the bone marrow is inhibited. Clinically, the main manifestations are skin, mucous membrane or visceral bleeding. Blood oozing, menorrhagia in women or severe vomiting, hemoptysis, blood in the stool, hematuria and other symptoms, complicated by intra...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/533
Inventor 何杨朱明清赵赟霄吴庆宇阮长耿
Owner 苏州博赛生物医药有限公司
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