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Injectable breast repair material, its preparation method and its application

A repair material and breast technology, which is applied in the fields of breast repair materials, plastic surgery and plastic surgery materials, can solve the problems of low fat survival rate, fat cell necrosis, cell membrane rupture, etc., to improve survival rate, leave no scars, trauma and pain less effect

Inactive Publication Date: 2011-12-21
王影
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the material is autologous tissue, theoretically there is no "rejection" problem, and there is no complication of non-autologous tissue; but fat is easily absorbed by the body, the survival rate of fat is low, and unsurviving fat is easy Infectious calcification and other lesions
In the autologous fat transplantation operation, due to the failure to establish blood supply in time, a large number of fat cells are necrotic, cell membranes are ruptured, and fat droplets leak, which is the main reason for the low survival rate of fat

Method used

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  • Injectable breast repair material, its preparation method and its application
  • Injectable breast repair material, its preparation method and its application
  • Injectable breast repair material, its preparation method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Extraction, Purification and Culture of Autologous Adipose Stem Cells

[0024] (1) After removing redundant tissues such as blood vessels in the obtained adipose tissue (30 ml), use

[0025] Rinse 2-3 times with antibiotic D-PBS (Sigma, USA);

[0026] (2) Add 2mg / ml type I collagenase (Worthington, USA) to the washed fat

[0027] company) at 37°C for 60-90 min, complete medium (DMEM+10% FBS, both from Gibco, USA) to stop digestion;

[0028] (3) 200-mesh sieve to filter the digested product of human adipose tissue;

[0029] (4) After filtration, centrifuge at 1500 rpm for 10 min, resuspend the complete medium,

[0030] Trypan blue (Sinopharm Group) staining and counting;

[0031] (5) in complete medium with 1 × 10 5 / cm 2 The density of human adipose stem cells was seeded in

[0032] T25 plastic culture bottle (Corning, USA) and cultured in a 37°C, 5% carbon dioxide incubator;

[0033] (6) When the human adipose stem cells grow to about 85%, the cells a...

Embodiment 2

[0038] Example 2 Using the breast repair material of the present invention for breast enhancement

[0039] (1) The patient was 62 years old, and 100 ml of adipose tissue was extracted from the patient, and the adipose stem cells were extracted, purified, and cultured according to Example 1.

[0040] (2) After the first extraction of adipose tissue, the second liposuction operation was performed 40 days later to obtain 550ml of adipose tissue. In the laboratory, the adipose tissue was washed, centrifuged at low speed, degreased, dehydrated, and fibrous connective tissue removed. , to obtain complete fat particles.

[0041] (3) The adipose stem cells cultured in step (1) were collected and evenly mixed into the fat particles obtained in step (2) to obtain an injectable complex.

[0042] (4) The injectable compound obtained in step (3) is injected by the plastic surgeon into the adipose tissue, postmammary space, and subcutaneous tissue of the patient's breast through multi-tunn...

Embodiment 3

[0045] Example 3 Using the breast repair material of the present invention for breast asymmetry repair

[0046] (1) The patient was 41 years old, and 80 ml of adipose tissue was extracted from the patient, and the adipose stem cells were extracted, purified, and cultured according to Example 1.

[0047] (2) After the first adipose tissue extraction, the second liposuction operation was performed 26 days later to obtain 300ml of adipose tissue. In the laboratory, the adipose tissue was washed, centrifuged at low speed, degreased, dehydrated, and fibrous connective tissue removed. , to obtain complete fat particles.

[0048] (3) The adipose stem cells cultured in step (1) were collected and evenly mixed into the fat particles obtained in step (2) to obtain an injectable complex.

[0049] (4) The injectable compound obtained in step (3) is injected by the plastic surgeon into the adipose tissue, postmammary space, and subcutaneous tissue of the patient's breast through multi-tun...

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Abstract

The invention provides an injectable breast repair material, which is characterized in that the material includes autologous fat stem cells and autologous fat particles, wherein the number of fat stem cells contained in every 100ml of fat particles is 106-108. The invention also provides a preparation method and application of the injectable breast repair material. The cells used in the present invention are autologous adipose stem cells isolated from the patient's own adipose tissue, thus avoiding ethical controversy and immune rejection. The fat particles containing autologous adipose stem cells used in the present invention can be injected into the patient's body through a syringe, and can be injected into any part of the patient's breast except glands according to the shaping needs, and the application is very simple.

Description

technical field [0001] The invention relates to a material suitable for plastic surgery and cosmetic surgery, in particular to an injectable breast repair material, its preparation method and application, belonging to the field of tissue engineering. Background technique [0002] There is a huge clinical demand for breast repair, including breast augmentation, repair of breast asymmetry, loose and sagging breasts after breastfeeding, and repair after mastectomy. At present, there are mainly the following methods for breast repair. [0003] Implantation of prosthesis: Breast augmentation with prosthesis has the advantages of good effect, natural and beautiful shape, and realistic hand feeling. The disadvantages are relatively large surgical trauma, slow recovery, and high hardening rate. The body's immune response, capsular contracture, and breast hardening are common complications. Infection, improper position, etc. are also potential risks of implant implantation for brea...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/36
Inventor 王影徐妍
Owner 王影
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