Injectable breast repair material, its preparation method and its application

A repair material and breast technology, which is applied in the fields of breast repair materials, plastic surgery and plastic surgery materials, can solve the problems of low fat survival rate, fat cell necrosis, cell membrane rupture, etc., to improve survival rate, leave no scars, trauma and pain less effect

Inactive Publication Date: 2011-12-21
王影
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the material is autologous tissue, theoretically there is no "rejection" problem, and there is no complication of non-autologous tissue; but fat is easily absorbed by the body, the survival rate of fat is low, and unsurviving fat is easy Infectious

Method used

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  • Injectable breast repair material, its preparation method and its application
  • Injectable breast repair material, its preparation method and its application
  • Injectable breast repair material, its preparation method and its application

Examples

Experimental program
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Example Embodiment

[0023] Example 1 Extraction, purification and culture of autologous adipose stem cells

[0024] (1) After removing the excess tissues such as blood vessels in the obtained fat tissue (30 ml),

[0025] Rinse 2-3 times with antibiotic D-PBS (American Sigma Company);

[0026] (2) Add 2mg / ml type I collagenase to the washed fat (Worthington, USA)

[0027] Company) digested at 37°C for 60-90 min, complete medium (DMEM+10% FBS, all from Gibco, USA) to terminate the digestion

[0028] (3) 200 mesh screen filters the digested products of human adipose tissue;

[0029] (4) After filtering, centrifuge at 1500 rpm for 10 min, resuspend in complete medium,

[0030] Trypan blue (China National Pharmaceutical Group) is counted after dyeing;

[0031] (5) 1×10 5 / cm 2 Inoculate human adipose stem cells in

[0032] In a T25 plastic culture bottle (Corning, USA), and place it in a 37°C, 5% carbon dioxide incubator;

[0033] (6) When the human adipose stem cells grow to about 85%, cell passage is carried out. ...

Example Embodiment

[0038] Example 2 Using the breast repair material of the present invention for breast augmentation

[0039] (1) The patient was 62 years old, and 100 ml of adipose tissue was extracted from the patient, and adipose stem cells were extracted, purified, and cultured according to Example 1.

[0040] (2) After the first adipose tissue extraction, after 40 days for the second liposuction operation, 550ml adipose tissue was obtained. The adipose tissue was washed, centrifuged at a low speed, deoiled and watered, and fibrous connective tissue was removed in the laboratory. , To obtain complete fat particles.

[0041] (3) Collect the adipose stem cells cultured in step (1) and mix them evenly into the fat particles obtained in step (2) to obtain an injectable compound.

[0042] (4) The injectable compound obtained in step (3) is injected into the fat tissue, posterior breast space, and subcutaneous tissue of the patient's breast by the plastic surgeon through multiple tunnels and multiple lev...

Example Embodiment

[0045] Example 3 Application of the breast repair material of the present invention to breast asymmetric repair

[0046] (1) The patient was 41 years old, and 80 ml of the patient's adipose tissue was extracted, and the adipose stem cells were extracted, purified, and cultured according to Example 1.

[0047] (2) After the first adipose tissue extraction, a second liposuction operation was performed after 26 days to obtain 300ml adipose tissue. The adipose tissue was washed, centrifuged at a low speed, deoiled and watered, and fibrous connective tissue was removed in the laboratory. , To obtain complete fat particles.

[0048] (3) Collect the adipose stem cells cultured in step (1) and mix them evenly into the fat particles obtained in step (2) to obtain an injectable compound.

[0049] (4) The injectable compound obtained in step (3) is injected into the fat tissue, posterior breast space, and subcutaneous tissue of the patient's breast by the plastic surgeon through multiple tunnels...

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Abstract

The invention provides an injectable breast repair material, which is characterized in that the material includes autologous fat stem cells and autologous fat particles, wherein the number of fat stem cells contained in every 100ml of fat particles is 106-108. The invention also provides a preparation method and application of the injectable breast repair material. The cells used in the present invention are autologous adipose stem cells isolated from the patient's own adipose tissue, thus avoiding ethical controversy and immune rejection. The fat particles containing autologous adipose stem cells used in the present invention can be injected into the patient's body through a syringe, and can be injected into any part of the patient's breast except glands according to the shaping needs, and the application is very simple.

Description

technical field [0001] The invention relates to a material suitable for plastic surgery and cosmetic surgery, in particular to an injectable breast repair material, its preparation method and application, belonging to the field of tissue engineering. Background technique [0002] There is a huge clinical demand for breast repair, including breast augmentation, repair of breast asymmetry, loose and sagging breasts after breastfeeding, and repair after mastectomy. At present, there are mainly the following methods for breast repair. [0003] Implantation of prosthesis: Breast augmentation with prosthesis has the advantages of good effect, natural and beautiful shape, and realistic hand feeling. The disadvantages are relatively large surgical trauma, slow recovery, and high hardening rate. The body's immune response, capsular contracture, and breast hardening are common complications. Infection, improper position, etc. are also potential risks of implant implantation for brea...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/36
Inventor 王影徐妍
Owner 王影
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