Sterilization composition
A composition and compound technology, applied in the directions of fungicides, biocides, biocides, etc., to achieve the effects of general preservation and yield increase, lasting drug effect, and delaying crop drug resistance
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example 1
[0043] (1) Experimental object:
[0044] Cucumber downy mildew pathogen was isolated and purified from cucumber downy mildew diseased leaves in Shouguang cucumber greenhouse in Shandong Province, and cultured and preserved on PDA slopes in a 4°C refrigerator.
[0045] Test medium:
[0046] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0047] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0048] (2) Experimental medicine:
[0049] 90% cyflufenamide technical product, Germany Bayer Crop Science Co., Ltd., 99% cyprodinil technical product, Japan Ishihara Sangyo Co., Ltd.
[0050] Mix and match settings:
[0051] The content percentages of 19% cyflufenamide and cyprodinil are 3:16, 5:14, 7:12, 9:10, 11:8, namely 3%+16%, 5%+14%, 7% +12%, 9%+10%, 11%+8%, a total of 5 mixed combinations.
[0052] (3) Test method:
[0053] Toxicity determination of single ag...
example 2
[0071] Biological Assay Example 2: Indoor Toxicity Determination of Cyflufen and Azoxystrobin Compound on Cucumber Anthracnose Pathogen.
[0072] 97% azoxystrobin technical product, Lanzhou Tradewind Forest Fine Chemical Co., Ltd.
[0073] Cucumber anthracnose pathogen was isolated and purified from cucumber anthracnose-infected leaves in Shouguang cucumber shed, and cultured and preserved on PDA slant at 4°C refrigerator.
[0074] Toxicity assays for single-dose cycloflufen and azoxystrobin, EC 50 The values were 3.07 mg / l and 7.42 mg / l, respectively.
[0075] Mix and match settings:
[0076] The content percentages of 17% fluflufenamide and azoxystrobin are 3:14, 5:12, 7:10, 9:8, 11:6, that is, 3%+14%, 5%+12%, 7%+ 10%, 9%+8%, 11%+6% total 5 mixing combinations.
[0077] Test method is the same as biological assay embodiment 1
[0078] Experimental results and analysis:
[0079] Table 3 Toxicity test of the combination of fluflufen and azoxystrobin against cucumber an...
example 3
[0085] Biological Assay Example 3: Indoor Toxicity Determination of Cyflufenamide and Pyrimethanil Compound to Cucumber Botrytis.
[0086] 98% pyrimethanil technical, Jiangsu Changlong Agrochemical Co., Ltd.
[0087] Cucumber gray mold fungus was isolated and purified from cucumber gray mold diseased leaves in Shouguang cucumber greenhouse in Shandong Province, and cultured and preserved on PDA slopes in a 4°C refrigerator.
[0088] Toxicity Assays for Single-Agent Cyflufenamide and Pyrimethanil, EC 50 The values were 4.16 mg / l and 6.88 mg / l, respectively.
[0089] Mix and match settings:
[0090] The content percentages of 20% fluvafen and pyrimethanil are 4:16, 6:14, 8:12, 10:10, 12:8, that is, 4%+16%, 6%+14%, 8%+ 12%, 10%+10%, 12%+8% total 5 mixing combinations.
[0091] Test method is the same as biological assay embodiment 1
[0092] results and analysis:
[0093] Table 5 Combined toxicity of flufenamide and pyrimethanil compound against Botrytis cinerea
[0094]...
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