Controlled release microecological preparation for water quality improvement and preparation method thereof
A micro-ecological preparation, water quality improvement technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as water quality improvement, and achieve improved water quality, strong adsorption and protection, and strong surface adsorption. Effect
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Embodiment 1
[0016] The preparation of embodiment 1 controlled-release probiotics
[0017] 1. Seed culture of Bacillus subtilis
[0018] The seed medium of Bacillus subtilis is: 0.5% sucrose, MgSO 4 0.02%, CaCO 3 0.5%, K 2 HPO 4 0.02%, pH7.0~7.2. The volume of 500ml triangular bottle is 100ml, sterilized at 121°C for 20 packs, cooled and then connected to a slope ring, cultured on a shaking table, the rotating speed of the shaking table is 200 rpm, and the cultivation temperature 37°C, incubation time 28 hours.
[0019] 2. Production of Bacillus subtilis
[0020] The liquid medium of Bacillus subtilis is 1% sucrose, 0.02% yeast extract, MgSO 4 0.01%, Na 2 HPO 4 0.01%, CaCO 3 0.1%, pH 7.0. After the fermenter was sterilized for 1 hour in advance, pour the culture medium into a 1-ton tank, fill with 800L fermentation broth, sterilize at 121-125°C for 1 hour, cool to 30-35°C, and inoculate.
[0021] The fermentation conditions are as follows: the fermentation temperature is 37°...
Embodiment 2
[0026] The preparation of embodiment 2 controlled-release probiotics
[0027] The preparation method is the same as that described in Example 1, except that the ratio of the composite bacteria is different. Controlled release Bacillus subtilis powder 60%, Bacillus cereus 15%, Lactic acid bacteria 10%, Yeast 15%.
Embodiment 3
[0028] The preparation of embodiment 3 controlled-release probiotics
[0029] The preparation method is the same as that described in Example 1, except that the ratio of the composite bacteria is different. Controlled release Bacillus subtilis powder 75%, Bacillus cereus 12%, Lactic acid bacteria 7%, Yeast 6%.
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