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Preparing device of cell sheet for cold and hot regulation of temperature sensitive gel by utilizing semiconductor refrigerating sheets

A temperature-sensitive gel and a technology for preparing a device, which are used in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc. Long time and other problems, to achieve the effect of simple and reliable preparation device, reducing cell damage, and timely monitoring and improving

Active Publication Date: 2012-01-25
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the realization and control of temperature changes in the process of cell sheet preparation is still a difficult problem. At present, most of them are realized by reducing the indoor environment, which takes a long time, and it is not easy to accurately control the temperature-sensitive hydrogel in the temperature-sensitive culture dish. low temperature can easily cause damage to cells

Method used

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  • Preparing device of cell sheet for cold and hot regulation of temperature sensitive gel by utilizing semiconductor refrigerating sheets
  • Preparing device of cell sheet for cold and hot regulation of temperature sensitive gel by utilizing semiconductor refrigerating sheets
  • Preparing device of cell sheet for cold and hot regulation of temperature sensitive gel by utilizing semiconductor refrigerating sheets

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Experimental program
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Effect test

Embodiment 1

[0021] Depend on figure 1 with 2 It can be seen that in this embodiment, the fixed frame (1) and the controller (6) are integrated, and all wires are integrated in the device, which has a compact structure. When the bottom area of ​​the culture vessel (3) is small and the required cooling capacity is not large, a hollow hole (9) is provided at the bottom of the fixed frame (1) to expose the bottom surface of the semiconductor cooling chip (2) to facilitate the use of air to dissipate heat.

Embodiment 2

[0023] Depend on image 3 It can be seen that in this embodiment, the fixed frame (1) and the controller (6) are integrated, and all wires are integrated in the device, which has a compact structure. When the bottom area of ​​the culture vessel (3) is large and the required cooling capacity is large, the bottom of the fixed frame (1) is processed into heat sinks (10) to increase the heat dissipation area.

Embodiment 3

[0025] Depend on Figure 4 It can be seen that in this embodiment, the fixed frame (1) and the controller (6) are separated separately, and the semiconductor refrigerator (2) and the temperature sensor (5) are connected to the controller (6) through wires (11) and wires (12) respectively. , the cooling and heating of the semiconductor cooling chip (2) can be remotely controlled by the controller (6). The fixed frame (1) can not only adopt figure 1 with figure 2 The square shown can also be processed into a circle or other shapes when the culture vessel (3) is a circle or other shapes. When the bottom area of ​​the culture vessel (3) is small and the required cooling capacity is not large, a hollow hole (9) is provided at the bottom of the fixed frame (1) to expose the bottom surface of the semiconductor cooling chip (2) to facilitate the use of air to dissipate heat.

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Abstract

The invention discloses a preparing device of a cell sheet for the cold and hot regulation of a temperature sensitive gel by utilizing semiconductor refrigerating sheets, which comprises a fixing frame (1). The semiconductor refrigerating sheets (2) are arranged on the fixing frame; the semiconductor refrigerating sheets (2) are coated with heat-conducting oil and are in close connection with a culture utensil (3); the semiconductor refrigerating sheets (2) are connected in parallel or in series and are connected with a controller (6) connected with a power supply through a conducting wire; atemperature sensitive gel (4) coats the bottom of the inner wall of the culture utensil (3); a temperature sensor (5) is arranged at the bottom of the outer wall of the culture utensil; and the temperature of the temperature sensitive gel (4) is regulated through the semiconductor refrigerating sheets (2) in the culture utensil, so as to achieve the preparation of a tissue engineering cell sheet.The device has a simple and compact structure and a quick temperature change speed, and further can be monitored in a timely way.

Description

technical field [0001] The invention relates to a cell sheet preparation device, in particular to a cell sheet preparation device which utilizes a semiconductor refrigerating sheet to regulate temperature-sensitive gel cold and heat. Background technique [0002] Tissue engineered cell sheets separate cultured cells from the culture surface to form a complete sheet structure containing extracellular matrix through tissue engineering methods, which is a new technology for harvesting and transferring seed cells in tissue engineering. Tissue engineered cell sheets contain important cell surface proteins such as ion channels, growth factor receptors and connexins, which can avoid the side effects caused by the use of carriers such as scaffolds. It is widely used in the repair of air leakage and so on. [0003] In the early days, some scholars evenly inoculated cells in glass culture dishes, and when all the colonies were basically connected into sheets, they were peeled off fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 吕永钢邹杨杨力
Owner CHONGQING UNIV
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