Platelet aggregation using a microfluidics device
A technology of platelet aggregation and microfluidics, applied in the direction of using thermal variables to measure fluid velocity, applying thermal effects to detect fluid flow, fluid controllers, etc.
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example 1
[0232] Example 1 Blood Perfusion Through Step Geometry
[0233] Figure 11 a A series of representative photomicrographs (40× magnification) of human (hirudin-anticoagulated) blood perfusion through a microshear gradient device consisting of 100 μm inflow (entrance) width, 90° constriction angle θ c , 10μm gap height, 60° expansion angle θ e , 700μm expansion / exit width composition. The gray arrows indicate the initial aggregation point [t=12sec], and the black arrows indicate the extent of thrombus growth in the expanded area (n=3 experiments).
[0234] Figure 11 b is the result (velocity v displacement (displacement) diagram) obtained from the computational fluid dynamics (CFD) simulation, showing that the platelet (particle) moves 1 μm (1 / 2 the disc platelet diameter) from the surface of the microchannel wall geometry in (a) ) changes in speed. For linear microchannel part (1,800.s -1 In laminar flow), the platelets move at a uniform velocity throughout their path le...
example 2
[0239] Example 2 The flow rate dependency of the interaction of two step geometries
[0240] Figure 12a Included are representative aggregation records depending on flow rate (Q = 2, 4, 6, 8 μl / min) through a microshear gradient device consisting of 100 μm inflow / inlet width, 90° constriction angle (θ c ), 20μm gap height, 30° expansion angle (θ e ), 700 μm expansion / exit width composition.
[0241] Figure 12b For representative aggregation recordings, depending on flow rate (Q = 2, 4, 6, 8 μl / min) through a microshear gradient device consisting of 100 μm inflow width and 90° constriction angle θ c , 20μm gap height, 90° expansion angle θ e , consisting of 700 μm expansion width.
[0242] Analysis of the dependence of flow rate (Q = 2, 4, 6, 8 μl / min) was detected in two step geometry configurations with divergence angles of 30° and 90° (see respectively Figure 12a and Figure 12b ). In both geometry configurations, the size of the aggregates decreased significantly...
example 3
[0243] Example 3 spherical geometry
[0244] Figure 13a includes a DIC image box, showing the use of 10,000.s -1 γ Nature and extent of discoid platelet aggregation on the downstream side of the VWF-coated spherical geometry after perfusion of human whole blood (pretreated with 100 μM MRS2179, 10 μM 2-MeSAMP, and 10 μM indomethacin) (n=5).
[0245] Figure 13b shows the size of the average discoid platelet aggregate (surface area in μm 2 denoted), depending on the downstream low τ x，y Pit (surface area of zone 3 in μm 2 Indicates), showing τ≤30.4Pa (n=3).
[0246] Figure 13c is using 10,000.s -1 γ, size of disc-shaped platelet aggregates on the downstream face of the spherical geometry coated with 5 μm VWF; control, hirudin anticoagulated whole blood; anti-α IIb beta 3 , hirudin anticoagulated whole blood treated with 30 μg / ml c7E3Fab for 10 minutes before blood perfusion; anti-GPIb, hirudin anticoagulated whole blood treated with 50 μg / ml anti-GPIb blocking IgGALMA12 f...
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