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Peach gum polysaccharide, its extract, preparation method and application thereof

A technology of peach gum polysaccharides and extracts, applied in the fields of application, food preparation, food science, etc., can solve the problems of narrow application fields, decomposition of peach gum polysaccharides, difficult industrial production, etc., and achieve high viscosity, good stability, high The effect of molecular weight

Active Publication Date: 2013-06-05
SHANGHAI HUIWEN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the backward peach gum processing technology in the prior art, which easily causes peach gum polysaccharides to decompose during the processing process, resulting in a decline in the quality of the peach gum extract, and the peach gum extract produced by this process Containing a large number of impurities, narrow application fields, difficult industrial production and other defects, a peach gum polysaccharide and its extract, as well as its preparation method and application are provided

Method used

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  • Peach gum polysaccharide, its extract, preparation method and application thereof
  • Peach gum polysaccharide, its extract, preparation method and application thereof
  • Peach gum polysaccharide, its extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0054] The model of the dialysis bag in Example 4 is R-25--3.5K, purchased from Jinan Haobo Biotechnology Co., Ltd.

[0055] The test methods involved in the following examples are:

[0056] One, the determination method of peach gum polysaccharide content in the peach gum polysaccharide extract is:

[0057] Using xylose as a standard, the content of peach gum polysaccharides in peach gum polysaccharide extracts was determined by the phenol-sulfuric acid method (Zhang Weijie. Biochemical Research Technology of Glycosomes [M]. Zhejiang University Press. 2003).

[0058] (1) draw xylose standard curve:

[0059] Using xylose as a standard, prepare standard solutions respectively. Take xylose standard solution, add 6% phenol solution, add concentrated sulfuric acid, let stand for 10 minutes, shake well, carry out color reaction until the solution is orange-red. Measure the absorbance value at a wavelength of 480nm, and use water after the same color development operation as a bl...

Embodiment 1

[0091] Example 1 peach gum polysaccharide extract

[0092] The preparation method of peach gum polysaccharide extract is:

[0093] (1) Pulverization: the natural raw peach gum is pulverized, and all pass through a 20-mesh standard sieve to obtain natural raw peach gum coarse powder, which is set aside. Said above-mentioned natural raw peach gum coarse powder 25g, pours in the 2000ml there-necked flask, adds pure water 125ml (5ml / g peach gum coarse powder) wherein, forms peach gum suspension;

[0094] (2) Biological enzymatic hydrolysis: heat the peach gum suspension to 40°C, add 0.63g of alkaline protease (0.005g / ml peach gum suspension) to it, keep warm and stir for 1h, carry out biological enzymatic hydrolysis, and obtain the material Liquid 1;

[0095] (3) Low-temperature hydrolysis: raise the temperature of feed liquid 1 to 40°C, add 12.5 g of solid sodium hydroxide (0.1 g / ml feed liquid 1) to it, keep warm and stir for 1 hour, and carry out low-temperature hydrolysis to...

Embodiment 2

[0103] Example 2 peach gum polysaccharide extract

[0104] The preparation method of peach gum polysaccharide extract is:

[0105] (1) Grinding: Cut and crush 30.0 g of wet peach gum picked from the tree trunk, add 700ml of pure water (23.3ml / g wet peach gum) to it, swell in pure water at 50°C for 24 hours, and grind to obtain peach The glue suspension is ready for use.

[0106] (2) Biological enzymatic hydrolysis: Pour the above-mentioned peach gum suspension into a 2000ml three-neck flask, heat to 50°C, add 22.5g of neutral protease (0.032g / ml peach gum suspension), keep warm and stir for 3h , carry out biological enzymolysis, obtain feed liquid 1;

[0107] (3) Low-temperature hydrolysis: raise the temperature of feed liquid 1 to 50° C., add 140 g of solid sodium hydroxide (0.2 g / ml feed liquid 1) to it, keep warm and stir for 3 hours, and carry out low-temperature hydrolysis to obtain feed liquid 2;

[0108] (4) Adjust the pH: add 1500ml of pure water (50ml / g wet peach g...

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Abstract

The invention discloses a peach gum polysaccharide, comprising 70-98 wt% of neutral total sugar and no nucileic acid, protein, tannin, dextrin, starch and other impurities, wherein, the content of glucuronic acid in the neutral total sugar accounts for 5-30 % of the content of the peach gum polysaccharide, the average molecular weight of the peach gum polysaccharide is more than 3000 dalton, and an aqueous solution of the peach gum polysaccharide has a dynmaic viscosity of 0.5-4.0 pa. s, a pH value of 4.0-6.5, and a specific rotation defined in the description. The invention also provides an extractive of the peach gum polysaccharide, and a preparation method of the peach gum polysaccharide and the extractive, and an application as a stabilizing agent. The preparation method of the peach gum polysaccharide and the extractive can ensure that various physicochemical properties of the peach gum polysaccharide satisfy the requirements of use in food, medicines, cosmetics, and light industry field, keeps natural state of the peach gum polysaccharide, has no effluence on the product properties of the peach gum polysaccharide, ensures the safety, stability, validity and controllability of the product, and has a wide commercial prospect and application future.

Description

technical field [0001] The invention relates to a natural plant polysaccharide, in particular to a peach gum polysaccharide and an extract thereof, and also relates to a preparation method and application of the peach gum polysaccharide and an extract thereof. Background technique [0002] Peach gum polysaccharide has the effects of nourishing qi, harmonizing blood, and quenching thirst. It is good at promoting blood circulation, reducing swelling, and relieving pain. Gum polysaccharide can also be used as a new type of pharmaceutical excipient in the production of pharmaceutical preparations. It can also be used as a suspending agent, stabilizer, lubricant, microcapsule encapsulation material and film coating material. Peach gum polysaccharides are also edible, and the properties of thickening, emulsification, and solidification of peach gum polysaccharides and their extracts can be used to produce candy, cakes, dairy products, chocolate, peptone plastic food wrap, beverage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08L5/00A23L1/03A61K47/36A61K8/73A61Q19/00A23L29/00
Inventor 骆峰刘桂云周自华杨海延
Owner SHANGHAI HUIWEN BIO TECH
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