Leymus chinensis saline alkali stress induced 14-3-3 protein gene

Abstract

The invention relates to leymus chinensis saline alkali stress induced 14-3-3 protein gene, and belongs to the technical field of gene engineering. According to the present invention, a RACE method is adopted to carry out cloning to obtain a full-length sequence of leymus chinensis saline alkali stress induced 14-3-3 protein gene with strong saline alkali tolerant property; a Northern method and a genetic transformation method are adopted to detect the expression of the leymus chinensis saline-alkali stress induced 14-3-3 protein gene under the saline alkali stress, and analyze whether the leymus chinensis saline alkali stress induced 14-3-3 protein gene is related with the saline alkali tolerant property. The coding region of the 14-3-3 protein gene of the present invention comprises 786 bp, and codes 262 amino acids. Compared to other gramineous plants such as barley, wheat and the like, the 14-3-3 protein gene has higher homology, 10% difference exists in the DNA sequences of the 14-3-3 protein gene, 5% difference in the amino acid sequence of the 14-3-3 protein gene. The gene logging information and the related sequence information of the leymus chinensis 14-3-3 protein gene of the present invention are not reported. The Northern hybridization results of the gene of the present invention show that: the gene of the present invention is related with the saline alkali tolerant property of the plant. After the gene of the present invention is transformed into the ground-grow chrysanthemum, the transgenic ground-grow chrysanthemum can normally grow on the culture medium containing 100-200 mmol / L of NaCl, the growth conditions of the roots and the plants of the transgenic ground-grow chrysanthemum are better than the growth conditions of the roots and the plants of the untransformed ground-grow chrysanthemum, and the transgenic ground-grow chrysanthemum shows good saline alkali tolerant property.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a saline-alkali stress-induced protein gene of Leymus chinensis. Background technique [0002] It is an effective way to apply plant transgenic technology to the cultivation of plants with strong saline-alkali tolerance, and it has been reported that various genes have been patented. At present, the saline-alkali resistance genes used in molecular breeding of crops are mostly derived from non-saline model plants such as Arabidopsis thaliana and rice. Although there are reports of improving crop stress resistance through transgene overexpression, the transgenic efficiency and function There is still room for improvement. [0003] Most of the saline-alkali soils in my country are chloride salts (Cl - ) saline-alkali soil, which is mainly affected by salinization, that is, Na + stress to plants, and the salient feature of saline-alkali land in Northeast China is not only...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the salt-tolerant transgenic plants obtained through the transformation of the existing salt-tolerant genes have low salt-tolerant ability, and to clone a saline-alkali stress response gene of Leymus chinensis, a forage grass with strong salt-alkali resistance. (Leymus chinensis) Alkaline Stress-Induced 14-3-3 Protein Gene

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