Salinity and alkalinity stress-induced aquaporin PIP gene of Leymus chinensis

Abstract

The invention which relates to a salinity and alkalinity stress-induced aquaporin PIP gene of Leymus chinensis belongs to the technical field of genetic engineering. By utilizing an RACE method to clone in the invention, the full length sequence of a strong salinity and alkalinity resistance salinity and alkalinity stress response gene PIP protein gene of Leymus chinensis is obtained, the expression case of the PIP protein gene is detected through the Northern detection and the genetic transformation detection, and the expression case is used to analyze that whether the expression case is related with the salinity and alkalinity stress resistance. The length of the coding region of the obtained PIP protein gene is 879bp, and two hundred and ninety-two amino acids are coded. Compared with barley, wheat and the like which are Gramineae plants, the homology of Leymus chinensis is high, the DNA sequence difference is about 10%, and the amino acid sequence difference is less than 5%. No gene registration information and relevant sequence information reports of the PIP protein gene of Leymus chinensis are reported. Results of PIP protein gene Northern hybridization and gene chip technology researches of the expression of the salinity and alkalinity stress-induced aquaporin PIP gene of Leymus chinensis stressed by the salinity and the alkalinity show that the expression level of the PIP protein gene stressed by the salinity and the alkalinity is increased over two times, so the PIP protein gene is a salinity and alkalinity stress-related gene.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a saline-alkali stress-induced protein gene of Leymus chinensis. Background technique [0002] It is an effective way to apply plant transgenic technology to the cultivation of plants with strong saline-alkali tolerance, and it has been reported that various genes have been patented. At present, the saline-alkali resistance genes used in molecular breeding of crops are mostly derived from non-saline model plants such as Arabidopsis thaliana and rice. Although there are reports of improving crop stress resistance through transgene overexpression, the transgenic efficiency and function There is still room for improvement. [0003] Most of the saline-alkali soils in my country are chloride salts (Cl - ) saline-alkali soil, which is mainly affected by salinization, that is, Na + stress to plants, and the salient feature of saline-alkali land in Northeast China is not only...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the salt-tolerant transgenic plants obtained through the transformation of the existing salt-tolerant genes have low salt-tolerant ability, and to clone a saline-alkali stress response gene of Leymus chinensis, a forage grass with strong salt-alkali resistance. (Leymus chinensis) Salt-Alkaline Stress Induced Aquaporin PIP Gene

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