Application of atemisia gmelinii extract in preparing drugs for preventing liver injuries
A liver injury and drug technology, applied in the field of extracts of Artemisia japonica, can solve the problem of no particularly good method, and achieve the effect of significant curative effect and wide range of sources.
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[0013] 1. Separation of monomeric compounds
[0014] Take 1500g of Artemisia persicae, soak in water for 3 hours, heat for 2 hours, filter to obtain filtrate I, add water to the residue and heat for 1 hour to obtain filtrate II, combine filtrates I and II to obtain Artemisia persicae water extract. Add the water extract of Artemisia tennensis to the pretreated macroporous adsorption resin column, elute with distilled water, 50% ethanol, and 95% ethanol in turn, collect the 50% ethanol eluate, concentrate, freeze-dry to dryness, and extract the The 50% ethanol eluate of Artemisia tennensis was dissolved in water, extracted with dichloromethane and ethyl acetate in sequence to obtain dichloromethane extract, ethyl acetate extract and the remaining water-soluble part. An appropriate amount of the remaining water-soluble part was loaded on a pretreated reversed-phase silica gel column, and gradient elution was carried out in a methanol-water system. Observation and judgment base...
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