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Preparation method and application of sustained-release BIO/IGF (6-bromoindirubin-3'-oxime/insulin-like growth factor) composite hydrogel

A technology for compounding hydrogels and hydrogels, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of individual uniformity and cell absorption.

Active Publication Date: 2012-05-02
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method can promote the proliferation of cardiomyocytes, most of the compounds that promote the proliferation of cardiomyocytes are small molecular compounds, which require continuous maintenance of drug concentration to make them work

Method used

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  • Preparation method and application of sustained-release BIO/IGF (6-bromoindirubin-3'-oxime/insulin-like growth factor) composite hydrogel
  • Preparation method and application of sustained-release BIO/IGF (6-bromoindirubin-3'-oxime/insulin-like growth factor) composite hydrogel
  • Preparation method and application of sustained-release BIO/IGF (6-bromoindirubin-3'-oxime/insulin-like growth factor) composite hydrogel

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Experimental program
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specific Embodiment approach 1

[0013] Specific embodiment 1: The preparation method of the composite hydrogel of sustained-release BIO / IGF in this embodiment is carried out according to the following steps: 1. At 50°C, add the mixed solution composed of BIO and IGF to a mass concentration of 4% In ~6% gelatin solution, stir at 5000~6000r / min, add acetone dropwise at the same time, then add glutaraldehyde, and then centrifuge at 5000~7000r / min for 5~8h to obtain nanomicroparticles coated with BIO and IGF. 2. Add sodium periodate to the sodium alginate solution with a mass concentration of 0.5mg / mL under the condition of avoiding light. The volume ratio of sodium periodate to sodium alginate solution is 1:350-450, dissolve Then place it on a shaker at 4°C and shake it for 5-8 hours to obtain solution A, add ethylene glycol to solution A, the volume ratio of ethylene glycol to solution A is 1:150-250, and then shake it on a shaker 2 to 3 hours to obtain solution B, add sodium chloride to solution B, the volume...

specific Embodiment approach 2

[0016] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, the volume ratio of the mixture composed of BIO and IGF to the gelatin solution is 1:1000. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the volume ratio of gelatin solution to glutaraldehyde in step 1 is 5:1. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a preparation method and application of a sustained-release BIO / IGF (6-bromoindirubin-3'-oxime / insulin-like growth factor) composite hydrogel. The preparation method comprises the steps of: adding a mixed liquid of BIO and IGF to a gelatin solution, dropwise adding acetone under stirring, adding glutaric dialdehyde and centrifuging to obtain nano microsphere; adding sodium periodate to sodium alginate solution, shaking by a shaking table, adding ethylene glycol, shaking by the shaking table, adding sodium chloride, adding absolute ethanol to obtain a precipitate, dissolving the precipitate with deionized water, dialyzing, freeze-drying, and uniformly mixing freeze-dried powder with gelatin solution to obtain oxidized sodium alginate hydrogel; and centrifuging the nano microsphere to obtain a precipitate, adding the obtained precipitate to the sodium alginate hydrogel and uniformly mixing to obtain the sustained-release BIO / IGF composite hydrogel. The obtained sustained-release BIO / IGF composite hydrogel can be used for treating myocardial infarction. The prepared nano microsphere is a nano-scale medicament wrapping body, can be phagocytized by cells and can be easily absorbed by the cells.

Description

technical field [0001] The invention relates to a preparation method and application of a sustained-release BIO / IGF composite hydrogel. Background technique [0002] Myocardial infarction and the heart failure caused by it are chronic diseases with high morbidity and intractability in the world, and are the diseases with the highest mortality rate in industrial countries. Therefore, it is imminent to cure heart diseases. [0003] Since the death of myocardial cells after myocardial ischemia is the key problem leading to the loss of myocardial function, supplementing new cardiomyocytes is the preferred strategy for the treatment of myocardial infarction. At present, there are two main strategies for cardiomyocyte supplementation: (1) Relying on exogenous sources Research strategies for cell transplantation, such as embryonic stem cell transplantation, etc. Although the transplanted cells can replenish the missing cells in the damaged heart, there are ethical and moral issues...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K47/36A61K47/42A61K38/30A61P9/10A61K31/404
Inventor 田维明房蕊李钰
Owner HARBIN INST OF TECH
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