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Highly acidic chitosan-nucleic acid polyplex compositions

A composition, chitosan technology, applied in the directions of drug combination, sugar derivatives, genetic material components, etc., can solve the problem of difficulty in producing concentrated and stable dispersions of homogeneous chitosan-nucleic acid complexes

Inactive Publication Date: 2012-05-02
恩吉内股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] While high concentrations of nucleic acid are ideal for many applications, it is difficult to produce concentrated stable dispersions of homogeneous chitosan-nucleic acid complexes

Method used

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  • Highly acidic chitosan-nucleic acid polyplex compositions
  • Highly acidic chitosan-nucleic acid polyplex compositions
  • Highly acidic chitosan-nucleic acid polyplex compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0285] Embodiment 1: Small-scale diafiltration test for the first time

[0286] Table 2 describes the batch parameters for the diafiltration tests. The c150-pH4.0 formulation was used as starting material. A pH / Acetate adjustment step was added as a final step before fill & finish.

[0287] Table 2 Parameters and results

[0288]

[0289] 1. Measured after 17 hours at room temperature

[0290] 2. Measured after 6 hours at room temperature

Embodiment 2

[0291] Diafiltration method embodiment 2: the second small-scale diafiltration test

[0292] The second diafiltration run (Table 3) was a 3-fold larger batch and used the cl50-pH 4.0 formulation as starting material. A pH / Acetate adjustment step was added as a final step before fill & finish.

[0293] Table 3 Parameters and results

[0294]

Embodiment 3

[0295] Example 3: Small batches

[0296] A third diafiltration test was performed (Table 4). This batch also started with the c150-pH 4.0 formulation.

[0297] Table 4 parameters and results

[0298]

[0299]

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Abstract

The invention provides highly acidic chitosan-nucleic acid polyplex compositions. The compositions may be used to transfect cells in vitro and in vivo, and are particularly useful for transfecting cells of mucosal epithelia.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S.S.N. 61 / 165,442, filed March 31, 2009, which is expressly incorporated herein by reference in its entirety. technical field [0003] The invention relates to a strongly acidic chitosan-nucleic acid complex composition, and methods for its preparation and use. Background technique [0004] Chitosan is a non-toxic cationic copolymer of N-acetyl-D-glucosamine and D-glucosamine. Chitosan can form complexes with nucleic acids and has been used as a DNA delivery vehicle for transfected cells. [0005] Many biological applications of chitosan have involved the use of high molecular weight chitosan polymers. High molecular chitosan polymers of about hundreds of thousands to millions of Daltons are only soluble in acidic solutions. Dilute acetic acid is often used as a solvent for this polymer chitosan. [0006] Low-molecular-weight chitosan, on the order of tens of thousands of Da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87C12N15/10C07H21/00
CPCA61K47/4823A61K48/0041C12N15/87A61K47/61A61P11/00A61P11/06A61P13/10A61P29/00
Inventor J·高C·弗里特E·苏A·钟
Owner 恩吉内股份有限公司
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