Method for preserving pollen of asteraceae and allied plants

A technology for plant pollen and related genera, which is applied in the fields of plant preservation, botanical equipment and methods, application, etc., can solve the problems of decreased pollen viability, difficulty in hybrid breeding research, etc., and achieves the effect of a simple preservation method

Active Publication Date: 2013-12-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the pollen of Chrysanthemum and its related genera are preserved under normal conditions, the viability of the pollen will drop significantly, making it difficult to use for cross-breeding research.

Method used

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  • Method for preserving pollen of asteraceae and allied plants
  • Method for preserving pollen of asteraceae and allied plants
  • Method for preserving pollen of asteraceae and allied plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The fresh pollens of 10 relative genera of the genus Chrysanthemum chrysanthemum, shrub chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, white crystal chrysanthemum, safflower chrysanthemum, short tongue chrysanthemum, chrysanthemum chrysanthemum, and chrysanthemum were collected respectively, and the collection time was 2 The genus and species correspond to the flowering period, and choose sunny days between 10:00 and 14:00.

[0016] The collected fresh pollen was dried in the air for 1-2 hours to ensure its drying, and then some fresh pollen was taken for viability determination, and the rest of the fresh pollen was put into a tinfoil bag, and the tinfoil bag containing the pollen was stored at a low temperature of 4°C. The viability of pollen was detected every 2 months, and the determination was carried out 3 times continuously.

[0017] The detection of pollen viability is as follows: put the pollen sample into M...

Embodiment 2

[0024] The fresh pollens of 10 relative genera of the genus Chrysanthemum chrysanthemum, shrub chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, white crystal chrysanthemum, safflower chrysanthemum, short tongue chrysanthemum, chrysanthemum chrysanthemum, and chrysanthemum were collected respectively, and the collection time was 2 The genus and species correspond to the flowering period, and choose sunny days between 10:00 and 14:00.

[0025] Air-dry the collected fresh pollen for 1-2 hours to ensure it is dry, then take part of the fresh pollen for viability determination, put the rest of the fresh pollen into a foil bag, and store the tinfoil bag containing the pollen at -20°C , the viability of pollen was detected every 2 months, and the determination was carried out 3 times in a row.

[0026] The detection of pollen viability is as follows: put the pollen sample into ME3+200g·L -1 In PEG4000 medium, culture in the dark a...

Embodiment 3

[0033] The fresh pollens of 10 relative genera of the genus Chrysanthemum chrysanthemum, shrub chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, chrysanthemum chrysanthemum, white crystal chrysanthemum, safflower chrysanthemum, short tongue chrysanthemum, chrysanthemum chrysanthemum, and chrysanthemum were collected respectively, and the collection time was 2 The genus and species correspond to the flowering period, and choose sunny days between 10:00 and 14:00.

[0034] Air-dry the collected fresh pollen for 1-2 hours to ensure it is dry, then take part of the fresh pollen for viability determination, put the rest of the fresh pollen in a tinfoil bag, and store the tinfoil bag with the pollen at -80°C , the viability of pollen was detected every 2 months, and the determination was carried out 3 times in a row.

[0035] The detection of pollen viability is as follows: put the pollen sample into ME3+200g·L -1 In PEG4000 medium, culture in the dark at 20°...

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Abstract

The invention discloses a method for preserving pollen of asteraceae and allied plants, belonging to the field of hybridizing and breeding of chrysanthemums. The method comprises the following steps of: putting collected fresh pollen in a tinfoil paper parcel after airing for 1-2 h, preserving the pollen at -80-4 DEG C, and carrying out pollen activity detection one time every two months so as to evaluate the preserving activity of the pollen. Aiming at the asteraceae and allied wild species, a scientific and simple pollen preserving method is provided; the method is beneficial to developing the hybridizing and breeding work without time difference and geographic isolation; furthermore, the preserving temperature most suitable for maintaining the activity of the pollen is explored.

Description

technical field [0001] The invention relates to the field of crossbreeding of chrysanthemums, in particular to a method for preserving pollen of plants of the genus Chrysanthemum. Background technique [0002] Chrysanthemum originated in China and has been cultivated in China for more than 2,000 years. It is not only one of the top ten traditional famous flowers in China, but also one of the top four cut flowers and top ten potted flowers in the world. It has become the most important flower plant in the flower industry in the world. one. There are many varieties of chrysanthemums with rich flower colors, but there are still some deficiencies in resistance traits such as resistance to diseases and insect pests, heat resistance, and cold resistance, and ornamental traits such as plant type, etc., resulting in a serious reduction in its yield, quality and production efficiency. It also limits the geographical scope of its promotion and application. The wild plants of chrysan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
Inventor 滕年军黄至喆孙春青陈发棣房伟民陈素梅管志勇蒋甲福刘兆磊
Owner NANJING AGRICULTURAL UNIVERSITY
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