Taurohyodeoxycholic acid type II crystal and preparation method thereof
A technology of deoxycholic acid and suisulphonic acid, which is applied in the field of type II crystal of sulfodeoxycholic acid and its preparation, and in the field of compounds for treating cholesterol gallstones, and can solve problems such as not specifying the melting temperature of hyosodeoxycholic acid , to achieve the effect of ensuring uniform quality, good product fluidity and concentrated particle size distribution
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Embodiment 1
[0053] Example 1: Preparation of Hysodeoxycholic Acid Type II Crystals
[0054] Step 1: Preparation of hysulfodeoxycholic acid
[0055] In a three-necked flask equipped with a thermometer, a stirrer and a dropping funnel, stir and mix 40.0g of hyodeoxycholic acid, 10.5g of triethylamine and 450mL of dioxane, cool the solution in an ice-water bath, and add dropwise at 10°C Ethyl chloroformate 11.5g, continue to stir and react for 30min after dropping, then raise the temperature to 15°C and stir for 20min, filter the reaction solution, remove the triethylamine salt, and use the filtrate for later use; dissolve 16.0g of taurine in 100mL of 1.5mol / L NaOH aqueous solution, drop into the above filtrate, after dropping, stir and react in an ice-water bath for 3h, filter the reaction solution, pass the filtrate through a chromatography column equipped with a 732-type strong acid ion exchange resin, and control the outflow rate to 300mL / h; collect the effluent , the solvent was evapor...
Embodiment 2
[0060] Example 2: Preparation of Hysodeoxycholic Acid Type II Crystals
[0061] Step 1: Preparation of hysulfodeoxycholic acid
[0062] In a three-necked flask equipped with a thermometer, a stirrer and a dropping funnel, stir and mix 35.0g of hyodeoxycholic acid, 12.5g of triethylamine and 400mL of dioxane, cool the solution in an ice-water bath, and add dropwise at 12°C Ethyl chloroformate 13.5g, continue to stir and react for 25min after dropping, then raise the temperature to 16°C and stir for 25min, filter the reaction solution, remove the triethylamine salt, and set aside the filtrate; dissolve 12.0g of taurine in 120mL of 1.5mol / L NaOH aqueous solution, drop into the above filtrate, after dropping, stir and react in an ice-water bath for 3.5h, filter the reaction solution, pass the filtrate through a chromatographic column equipped with a 732-type strong acid ion exchange resin, and control the outflow rate to 285mL / h; collect the outflow Liquid, the solvent is evapora...
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