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Saccharifying enzyme type time-temperature indicator

A temperature indicator, glucoamylase technology, applied in the direction of material analysis by observing the influence of chemical indicators, a thermometer that gives an average/integral value, and analysis by making materials undergo chemical reactions, etc., can solve the problem of indicators There are problems such as lack of activation device, large amount of amylase, and difficulty in ensuring accuracy, so as to achieve obvious color rendering effect, simple reaction mechanism, and cost reduction

Inactive Publication Date: 2012-06-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The indicator lacks an activation device and must be put into use as soon as it is produced, otherwise the accuracy is difficult to guarantee
In addition, the amount of amylase required is relatively large, resulting in high cost

Method used

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  • Saccharifying enzyme type time-temperature indicator
  • Saccharifying enzyme type time-temperature indicator
  • Saccharifying enzyme type time-temperature indicator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] (1) Preparation of buffer solution: take 2.2g 12H 2 O??Na 2 HPO 4 , 6.84g 2H 2 O??NaH 2 PO 4 , add water to prepare 1L of buffer solution with pH=6.0;

[0016] (2) Preparation of dextrin solution: Take 4g of maltodextrin, add 100ml of buffer solution, stir well to dissolve all the dextrin, and then obtain a dextrin solution with a concentration of 4g / ml;

[0017] (3) Preparation of iodine solution: Add 0.5g of iodine and 1g of potassium iodide to a brown glass bottle, and then add 100ml of distilled water. After stirring for a period of time, place it in a cabinet to ensure a light-tight environment until the iodine particles are completely dissolved. That is, 0.5% iodine solution is obtained, and when used, it is diluted to obtain 0.1% iodine solution;

[0018] (4) Glucoamylase is produced by Wuxi Jiecheng Technology Co., Ltd., the concentration is 1.4g / ml, and the enzyme activity is 100000u / ml;

[0019] (5) Production of time-temperature indicator: Take 20ml of...

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Abstract

Provided is a saccharifying enzyme type time-temperature indicator. Dextrin solution and iodine solution are mixed to form mixing solution, the mixing solution and saccharifying enzyme liquid are independently packaged, and the mixing solution and the saccharifying enzyme liquid are mixed when in use. The purposes of indicating storage temperature and time are achieved by utilizing hydrolysis reaction of saccharifying enzyme and dextrin, using the iodine solution as indicating agents and displaying cumulative effects of time and temperature through color changes. The time-temperature indicator is stable in reaction system, obvious in color showing effect, low in cost and easy to practically apply.

Description

technical field [0001] The invention relates to the technical field of time-temperature indication, in particular to the technical field of indicating time and temperature by using the hydrolysis reaction of glucoamylase and dextrin. Background technique [0002] Frequent food quality and safety issues have aroused widespread concern. Food quality is not only affected by internal factors such as its own composition, but also affected by external environmental conditions such as temperature, light, and pressure, and quality and safety problems often appear during food production, storage, transportation, or shelf life, so controlling the external environment is a must. A very important link. Among all external environmental factors, temperature is a factor with high unpredictability. It often fluctuates due to environmental changes and deviates from the recommended temperature conditions, resulting in food spoilage even though it is within the shelf life. Therefore, an effe...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01K3/04
Inventor 钱静吴敬郑光临冯钦
Owner JIANGNAN UNIV
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