Application of miR-1 in production of preparation for treating primary glaucoma

A primary glaucoma, 1.mir-1 technology, applied in gene therapy, sensory diseases, genetic material components, etc., can solve problems such as affecting the patency of the aqueous humor outflow channel, blocking the trabecular meshwork, etc., to reduce the blockage The probability of trabecular meshwork mesh, reducing cell shedding, enhancing the effect of migratory activity

Inactive Publication Date: 2012-06-20
天津医科大学眼科中心
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Problems solved by technology

[0004] The cause of primary open-angle glaucoma may be related to the detachment and loss of trabecular meshwork cells from the trabecular scaffold, and abnormal changes such as detachment and loss of trabecular meshwork cells may block the trabecular meshwork and affect the outflow of aqueous humor The patency of the channel

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  • Application of miR-1 in production of preparation for treating primary glaucoma
  • Application of miR-1 in production of preparation for treating primary glaucoma
  • Application of miR-1 in production of preparation for treating primary glaucoma

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[0024] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0025] In the present invention, firstly, by detecting the expression level of miRNA-1 in trabecular meshwork cells under oxidative stress state, the change of miR-1 under simulated high intraocular pressure state is known. with 200 μM H 2 o 2 The trabecular meshwork cells were treated for 24 hours, RNA was extracted, and the expression of miR-1 was detected by real-time quantitative PCR. The results showed that compared with normal TM cells, miR-1 was significantly down-regulated in TM cells under oxidative stress, suggesting that miR-1 may play a regulatory role in the pathogenesis of POAG.

[0026] In the present invention, in order to understand the influence of miR-1 on cell function, we cloned the initial transcript pri-miR-1 of miR-...

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Abstract

The invention relates to an application of miR-1 in production of a preparation for treating primary glaucoma, an application of miR-1 in production of a reagent for inhibiting cell proliferation of human eye trabecular meshwork cells, an application of miR-1 in production of a reagent for inhibiting adhesion of human eye trabecular meshwork cells, an application of miR-1 in production of a reagent for enhancing migration capacity of human eye trabecular meshwork cells and an application of miR-1 in production of a reagent for inhibiting expression level of fibronectin in human eye trabecular meshwork cells. According to the invention, the regulation effects of miR-1 on the proliferation, migration and adhesion functions of the trabecular meshwork cells are proved on the basis of observing the expression condition of miR-1 in the trabecular meshwork cells under an oxidative stress state, and the targeting of miR-1 on FN (fibronectin) is further confirmed, and the treatment value of miR-1 on POAG (primary open angle glaucoma) is confirmed.

Description

technical field [0001] The invention belongs to the field of drugs for treating eye diseases, in particular to the application of miR-1 in the preparation of preparations for treating primary glaucoma. Background technique [0002] Primary open-angle glaucoma (POAG) is one of the common blinding eye diseases, characterized by progressive optic nerve damage and visual field defect, so far, its pathogenesis has not been fully understood, which limits the treatment means of development. [0003] MicroRNA (microRNA, miRNA) is a class of highly conserved non-coding single-stranded RNA molecules with a length of about 22 nt that post-transcriptionally regulate intracellular gene expression. Mature miRNA can act as a guide molecule, according to the principle of complementary base pairing, bind to the target mRNA and cause the splicing or translation inhibition of the target mRNA at the post-transcriptional level. Current research shows that miRNA not only participates in the nor...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P27/06
Inventor 汪建涛苏畅蒋少云
Owner 天津医科大学眼科中心
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