Method for improving hatching rate of artemia cysts

Abstract

The invention discloses a method for improving a hatching rate of artemia cysts. Hatching of the artemia cysts is carried out in a hatching culture medium, glucose oxidase and glucose are added into the hatching culture medium, the adding amount of the glucose oxidase is 10-100mg in per liter of hatching culture medium, the adding amount of the glucose is 30-80mg in per liter of hatching culture medium, and the density of the artemia cysts in the hatching culture medium is not larger than 6g of dry artemia cysts in per liter of hatching culture medium. The glucose oxidase added into the hatching culture medium impels diapause cysts to generate freely swimming artemia nauplius during a hatching period, and accordingly the hatching efficiency can be remarkably improved. By means of the method, the hatching amount of the artemia cysts in 24 hours can be over 90% of the hatching amount of the artemia cysts in 48 hours.

Description

technical field [0001] The invention relates to a production method for aquaculture live feed, in particular to a method for improving the hatching rate of Artemia eggs. Background technique [0002] Artemia nauplii are generally used as live feed in aquaculture, especially as live feed in the early stage of marine fish and shrimp larvae. There is generally no live feed such as Artemia nauplii on the market, only Artemia eggs, and the nauplii are obtained from the hatching of Artemia eggs. Hatching of Artemia eggs can be done in hatching medium. Artemia spore eggs are fully dehydrated and can survive for a long time or even several years in a dry environment with no oxygen and no light at low temperature. The long storage capacity and short time (within 24 hours) to produce free-swimming nauplii make it the most convenient source of live feed for aquaculture. [0003] Artemia eggs are collected from nature such as seaside salt pans and inland salt lakes. Among the collec...

AI Technical Summary

Problems solved by technology

However, the optimum parameters obtained by the above method are not of universal significance, because for different Artemia strains or different batches of Artemia eggs of the same strain, the optimum parameters are variable

[0005] The previous method was to add enough hydrogen peroxide at one time, and the medium environment would change drastically in a very short period of time. The disadvantages of using hydrogen peroxide solution to treat Artemia eggs are: 1) Different Artemia strains before use Or different batches of Artemia eggs of the same strain, a large number of experiments need to be carried out to determine the most suitable parameters, and in order to obtain the ideal hatching effect, the operation must be strictly controlled under this parameter, which brings great harm to the user. 2) in the implementation process, the Artemia ovum after hydration needs to be transferred to the hydrogen peroxide solution and the corresponding incubation medium, and the carrying out of these steps is very strict to the implementation time, which makes the implementation process extremely cumbersome and complicated; 3) Artemia eggs must be washed with water in time to remove residual hydrogen peroxide after passing through the hydrogen peroxide solution, and even need to be neutralized, which will inevitably increase the processing cost

Another treatment method using hydrogen peroxide is to directly add solid compounds capable of producing hydrogen peroxide, such as perborate, percarbonate, magnesium peroxide or calcium peroxide, to the incubation medium. The biggest disadvantage is that the pH value of the hatching medium changes greatly, and an excessively high pH value may cause the death of Artemia nauplii