High-throughput peptide synthesis method and device thereof

Abstract

The invention discloses a high-throughput peptide reaction device and a high-throughput peptide synthesis method. The device comprises a bottom box with an opening on the upper part and a reaction plate capable of covering the opening of the bottom box, and is characterized in that the side surface and / or the bottom surface of the bottom box is provided with at least one air intake port and one liquid discharging port, the reaction plate is provided with a plurality of reaction chambers arranged neatly, the reaction chambers respectively comprise an inlet and an outlet arranged in the cavity of the bottom box, and the outlets are sealed by sand cores. Multiple reaction chambers can be integrated on one plate, and the lower parts of the reaction chambers can be communicated with nitrogen and waste solution outlets for simultaneous implementation of multiple reactions. The volume of the reaction solution can be reduced, and multiple reactions can be carried out at the same time. The high-throughput peptide reaction device has the advantages of simple structure, convenient operation, high efficiency and the like.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a high-throughput polypeptide reaction device. Background technique [0002] Solid-phase peptide synthesis technology is a peptide synthesis technology that appeared in 1963, which is to transplant chemical reactions from pure liquid phase conditions to solid phase systems. In solid-phase peptide synthesis, solid-phase resin balls with activated ends are the core of the synthesis operation, and amino acids with protective groups react with the activated ends of the solid-phase resin balls as reactants, followed by simple filtration and washing operations , to remove excess reactants and solvents; then use chemical or physical means to remove the amino acid terminal protection, react the resin beads with another amino acid reagent, and so on, and finally use chemical means to peel off the synthesized polypeptide from the resin , the polypeptide of desired sequence can be obta...

AI Technical Summary

Problems solved by technology

[0009] At present, the steps of peptide synthesis are relatively cumbersome, usually using expensive synthesis equipment, or using glass column peptide synthesis reactors to manually complete each process one by one

The cost of synthesis equipment is too high, and only up to 10 peptides can be synthesized at a time through the glass column peptide synthesis reactor, which has cumbersome procedures, small reaction volume, and low efficiency

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