p28GANK monoclonal antibody and application thereof

A monoclonal antibody and hapten technology, applied in the field of medical bioengineering, can solve the problem of unclear progress of liver cancer

Inactive Publication Date: 2012-07-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies suggest that p28 GANK It may play an important role in the occurrence and development of liver cancer, but how it regulates the process of liver cancer is still unclear. So far, p28 GANK The expression level of can be directly used as a prognostic factor for the recurrence and metastasis of liver cancer, as well as related reports on individualized treatment of liver cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • p28GANK monoclonal antibody and application thereof
  • p28GANK monoclonal antibody and application thereof
  • p28GANK monoclonal antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Anti-human p28 GANK Preparation and purification of monoclonal antibodies

[0030] 1. Antigen Synthesis and Coupling

[0031] The synthetic design of antigenic polypeptides refers to GenBank, GeneID: 5716, and the specific sequences are NPDAKDHYEATAMHRC (SEQ ID NO: 1), CDEERVEEAKLLVSQ (SEQ ID NO: 2), YIENKEEKTPLQVAKGC (SEQ ID NO: 3), provided by Pocky Biotechnology (Shanghai) Co., Ltd. The company uses a solid-phase peptide synthesis method to synthesize peptides through an automatic peptide synthesizer.

[0032] The coupling of peptides and keyhole limpet hemocyanin (KLH) adopts the glutaraldehyde linkage described on page 865 of "Molecular Cloning Experiment Guide" 2nd Edition, written by Sambrook, translated by Jin Dongyan, Science Press, Beijing, 1999, p. Law.

[0033] 2. Preparation and Purification of Monoclonal Antibodies

[0034] 100 μg of the polypeptide KLH conjugate purified in the above 1 was diluted with PBS, mixed with an equal volume of com...

Embodiment 2

[0038] Example 2. Anti-human p28 GANK Identification and detection applications of monoclonal antibodies

[0039] 1. Western Blot detection application of monoclonal antibody

[0040] The denatured protein sample was subjected to SDS-PAGE protein electrophoresis; the protein was transferred to a nitrocellulose membrane (Schleicher & Schell company) by an electroporator; TBST containing 5% BSA was blocked for 1 h, washed once in TBST for 5 min; TBST containing 4% BSA was diluted with anti p28 GANK Monoclonal antibody primary antibody (1μg / ml), incubate for 2h, wash three times with TBST, 5min each time; dilute IR-800-labeled anti-mouse secondary antibody in TBST containing 4% BSA, incubate for 1h, wash four times with TBST, each time 5min; scan with an infrared laser scanner Odyssey. see results figure 1 . The results show that the monoclonal antibody can specifically recognize human p28 GANK molecular.

[0041] 2. Application of immunohistochemical detection of monoclon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to the technical field of medical bioengineering, in particular to a p28GANK monoclonal antibody and an application thereof to preparation of a prognostic assessment preparation for liver cancer or a liver cancer treatment medicament. The expression of the p28GANK protein is increased abnormally in a liver cancer cell, and the expression power of the p28GANK protein is closely relevant to patient prognosis, so that more attention is gradually paid to the action of the p28GANK protein in the development of the live cancer. The invention provides a monoclonal antibody of anti-human p28GANK, and further provides the application value of the monoclonal antibody of the anti-human p28GANK in the detection and judgment of prognostic analysis and individual-based treatment of a liver cancer patient. The invention provides a novel thought for the clinical individual-based treatment of the p28GANK.

Description

technical field [0001] The invention relates to the technical field of medical bioengineering, and is a p28 GANK A monoclonal antibody and its use for preparing a preparation for evaluating the prognosis of liver cancer or a drug for treating liver cancer. Background technique [0002] p28 GANK It is a protein present in human hepatocytes, usually in low levels in normal cells, but in high levels in liver cancer cells. Its coding frame contains 226 amino acids, the molecular weight is 25kDa, and it contains 6 repeated ankyrin sequences, GeneID: 5716. The inventors have prepared rabbit anti-human p28 GANK The polyclonal antibody is used in the differential diagnosis of liver cancer and has applied for a patent (see "p28 antibody for differential diagnosis of liver cancer and its preparation", patent number ZL 03116825.6). [0003] Recently it has been reported that p28 GANK Mainly by regulating endoplasmic reticulum stress (ER stress), NF-κB, P53 and Rb signaling pathway...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/18G01N33/53A61K39/395A61P35/00
CPCG01N2333/775C07K2317/34G01N33/57438C07K16/18C07K14/775G01N33/5748C07K16/303G01N2333/82A61P35/00
Inventor 王红阳陈瑶付静胡栋平罗韬董立巍
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap