Device and method for screening growth and migration behaviors of cells in three-dimensional matrixes at high throughput

Abstract

The invention discloses a device and a method for screening growth and migration behaviors of cells in three-dimensional matrixes at high throughput, which relate to the field of biology. An objective is arranged below an electric objective table; a culture room plate is arranged above the electric objective table; a glass slide is arranged at the bottom part in the culture room plate; a polydimethylsiloxane thin layer covers on the glass slide; a cell suspension layer is coated onto the polydimethylsiloxane thin layer; both sides of the cell suspension layer are respectively provided with an adhesive tape; and cover glass covers on the adhesive tapes. According to the device and the method for screening the growth and migration behaviors of the cells in the three-dimensional matrixes at the high throughput, the defect that the information of important behaviors of the cells under a three-dimensional state, such as growth and migration, cannot be provided by two-dimensional research is overcome. The invention provides a novel high-throughput three-dimensional screening device and a novel high-throughput three-dimensional screening method. The device not only is capable of monitoring various behaviors of the cells under the three-dimensional state in real time but also is capable of conveniently and efficiently screening the influences of various matrixes on the behaviors of the cells at the high throughput while screening the influences of different matrixes and different factors on the important behaviors of the cells under the three-dimensional state at the high throughput, such as the growth and the migration. Moreover, the device has low price and a simple structure.

Description

technical field [0001] The invention relates to the field of biology, in particular to a device and method for high-throughput screening of growth and migration behavior of cells in a three-dimensional matrix. Background technique [0002] Cell growth and migration are important steps in many important cell biological processes such as individual development, tissue repair, organ regeneration, and malignant metastasis of cancer. Cell growth and migration in two dimensions are quite different from those in three dimensions. At present, most of the devices for studying cell growth and migration are carried out on two-dimensional planar devices. Studies have shown that cells cultured in a three-dimensional state produce polarization and form grape-like clones, but cells cultured in a two-dimensional state do not. feature. In addition, cells exist in a three-dimensional form in the body, and various extracellular substrates and environments will affect cell growth and migration...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a high-throughput device and method for screening the growth and migration behavior of cells in a three-dimensional matrix, which overcomes the shortcomings of the existing two-dimensional research that cannot provide important behavioral information such as cell growth and migration in a three-dimensional state, and provides A new high-throughput three-dimensional screening device and method. The device not only monitors various behaviors of cells in three-dimensional state in real time, but also can high-throughput screen the effects of different substrates and different factors on important behaviors such as cell growth and migration in three-dimensional state. At the same time, it is convenient, efficient, and high-throughput to screen the effects of various substrates on cell behavior, and the device is cheap and simple in structure

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