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Promoter and application thereof

A DNA molecule and sequence technology, applied in applications, angiosperms/flowering plants, introduction of foreign genetic material using vectors, etc., can solve the problems affecting the expression level of gene function, affecting the improvement of rice grain weight and yield, etc., and achieve a wide range of applications. Effects of space and market prospects

Inactive Publication Date: 2013-10-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gene controlling rice grain weight is an important factor affecting rice grain weight, but its expression mode and its strength are directly regulated by the promoter of the gene itself, and the change of expression mode will affect the expression degree of gene function, thereby affecting the rice grain Increased weight and yield

Method used

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  • Promoter and application thereof
  • Promoter and application thereof
  • Promoter and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0020] Example 1. Acquisition of the promoter p-GS6 controlling rice grain weight gene and its effect verification

[0021] 1. Acquisition of the promoter p-GS6 controlling the rice grain weight gene

[0022] The inventors of the present invention discovered a new gene GS6 controlling rice grain weight, cloned and identified its upstream sequence, and obtained the promoter p-GS6.

[0023] We found that the repetition of the sequence TGAGAG at the transcription start site (TSS) +52bp plays an important role. We used the promoter prediction software Promoter scan to analyze the GS6 promoter. According to the promoter sequence analysis, the transcription start site There is no obvious TATAbox in the upstream, but the +4 site has typical characteristics of the initiation site. It is speculated that the gene is transcribed dependent on the initiator (Inr), and the results show that there are a large number of cis-actions around the TSS site elements, and GT1CONSENSUS (GTAAT), GTGA...

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Abstract

The invention discloses a promoter and application thereof. The promoter comprises a DNA sequence defined by a sequence 1 in a sequence table, a DNA sequence which has more than 90 percent of homology and same function with the DNA sequence defined by the sequence 1 in the sequence table, and a nucleotide sequence which can be hybridized with the DNA sequence defined by the sequence 1 in the sequence table under the highly strict condition. The promoter disclosed by the invention can be used for promoting expression of a foreign gene in plants and has important theoretical and practical meanings on paddy high-yield molecular breeding and wide application and market prospect in the agricultural field.

Description

technical field [0001] The present invention relates to a promoter and its application. Background technique [0002] Rice is an important food crop. Nearly 50% of the world's population feeds on rice. Improving rice yield is the goal that breeders are constantly pursuing. Tiller number, grain number per panicle and grain weight are the main components of rice yield. Among them, grain weight, as an important factor affecting rice yield, has gradually attracted people's attention. The gene controlling rice grain weight is an important factor affecting rice grain weight, but its expression mode and its strength are directly regulated by the promoter of the gene itself, and the change of expression mode will affect the expression degree of gene function, thereby affecting the rice grain Weight and yield increase. In the process of rice domestication, the number of alleles is constantly decreasing while satisfying human's demand for food, and the narrowness of genetic resourc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/82A01H5/00
Inventor 孙传清孙连军李晓娇朱作峰付永彩谭禄宾刘凤霞谢道昕
Owner CHINA AGRI UNIV