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Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof

A technology of arthrobacter and strain, applied in the fields of environmental protection and biotechnology agriculture, to achieve the effect of obvious denitrification and denitrification effect

Active Publication Date: 2012-07-25
郎溪品旭科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there are no public reports on the technology of isolating the nitrifying bacteria in the VBNC state in the sewage treatment system and the recovery of the cultivable VBNC state nitrifying bacteria in China.

Method used

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  • Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof
  • Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof
  • Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof

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Effect test

Embodiment 1

[0084] From the sewage biological treatment system, the preserved strain of VBNC Arthrobacter DSC4 strain was screened by resuscitation cultivable technology. The culturable VBNC Arthrobacter DSC4 strain was isolated from a sewage biological treatment system, and there were more than 90% of the general traditional isolates in the system. Methods It is difficult to obtain bacteria that are in a viable non-culturable (VBNC) state similar to dormancy, and the Arthrobacter DSC4 strain in a VBNC state is screened by using resuscitated culture technology. The preservation number of the strain is CCTCC No: M2011406, the preservation unit is: China Center for Type Culture Collection, the preservation address is: Wuhan University, Wuhan, China, and the preservation date is: November 21, 2011. Taxonomic designation: Arthrobacter DSC4 Arthrobacter sp. DSC4, this strain generally needs to be stored in ultra-low temperature freezing (minus 80 degrees). The DSC4 strain preserved at ultra-...

Embodiment 2

[0089] The cultivating and identifying method of the denitrification and denitrification effect of the culturable VBNC Arthrobacter DSC4 strain on the nitrate matrix, comprising the following steps:

[0090] ①Culturable VBNC Arthrobacter DSC4 strains were preserved, and under aseptic conditions, a small amount of strains were picked and transferred to NYGR medium, and activated at 30°C for 2-4 days;

[0091] ②Pick a small amount of activated cultured bacteria ①Inoculate into PYGN liquid medium for pre-cultivation at 30°C for 2 days;

[0092] ③Introduce 2% of the pre-culture bacteria solution into the Giltay medium, culture on a shaker at 30°C and 120 rpm for 2 days, observe the gas production and color change of the medium, and measure ammonia nitrogen and nitrous in the culture medium nitrogen, nitrate nitrogen and total nitrogen.

[0093] Ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen were respectively used by Nessler colorimetry, ultraviolet spectr...

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Abstract

The invention belongs to the fields of agricultural biotechnology and environmental protection and relates to a 'viable but non culturable (VBNC)' arthrobacterium strain which is obtained through separation by using recovery culturable technology, a recovering method of the strain and application of the strain. The preservation number of the strain is CCTCC No: M2011406; the preservation organization is China Center for Type Culture Collection; the preservation address is Wuhan University, Wuhan Province, China; and the preservation date is 25th Nov. 2011. The viable but non culturable (VBNC) arthrobacterium DSC4 can turn the color of the reaction liquid of a Giltay culture medium to dark blue from green and generate gas. At the same time, the conversion rate of nitrate nitrogen is detected to be 54.7 percent, the total nitrogen depletion rate is 52.9 percent, and residue of ammoniacal nitrogen and nitrite nitrogen is not detected. The strain has an obvious denitrification effect.

Description

technical field [0001] The invention belongs to the fields of biotechnology agriculture and environmental protection, and relates to a "viable non-culturable (VBNC)" Arthrobacter strain isolated by applying resuscitative culture technology ( Arthrobacter sp. DSC4) and the recovery method and application of this strain. Background technique [0002] The biological treatment of ammonia nitrogen-containing sewage is an extremely important technical measure in today's agriculture and environmental protection industries. Among them, the oxidation of ammonia nitrogen, the oxidation of nitrite nitrogen, the reduction of nitrate nitrogen and the denitrification of key technical links need more With the participation of various microorganisms, it degrades and transforms inorganic and organic nitrogen. Generally, the single-function nitrosifying bacteria that can be isolated traditionally such as Nitrosomonas, Nitrosococcus Species in the genus, nitrifying bacteria such as Nitro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/06
Inventor 丁林贤陈建荣金夷林冬珍
Owner 郎溪品旭科技发展有限公司
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