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Resurrecting culturable VBNC (viable but non-culturable) arthrobacterium ODSLC13 strain as well as resurrecting method and application thereof

A technology of Arthrobacter and strains, applied in the field of VBNC Arthrobacter ODSLC13 strain and its recovery, to achieve obvious denitrification and denitrification effects

Inactive Publication Date: 2014-09-03
丁林贤 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there are no public reports on the technology of isolating the nitrifying bacteria in the VBNC state in the sewage treatment system and the recovery of the cultivable VBNC state nitrifying bacteria in China.

Method used

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  • Resurrecting culturable VBNC (viable but non-culturable) arthrobacterium ODSLC13 strain as well as resurrecting method and application thereof
  • Resurrecting culturable VBNC (viable but non-culturable) arthrobacterium ODSLC13 strain as well as resurrecting method and application thereof

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Effect test

Embodiment 1

[0084] From the sewage biological treatment system, the preserved strain of VBNC Arthrobacter ODSLC13 strain was screened by resuscitation and culturable technology. The culturable VBNC Arthrobacter ODSLC13 strain was isolated from a sewage biological treatment system, and there were more than 90% of the general traditional isolation in the system. Methods It is difficult to obtain bacteria that are in a viable non-culturable (VBNC) state similar to a dormant state, and the Arthrobacter ODSLC13 strain in a VBNC state is screened by using the resuscitation cultivable technology. The preservation number of the strain is CCTCC No: M2014074, the preservation unit is: China Center for Type Culture Collection, the preservation address is: Wuhan University, Wuhan, China, and the preservation date is: March 05, 2014. The classification is named: Arthrobacter sp.ODSC13 ODSLC13, and the strain generally needs to be stored in ultra-low temperature freezing (minus 80 degrees). The ODSLC13...

Embodiment 2

[0089] The cultivating and identifying method of the denitrification and denitrification effect of the cultivable VBNC Arthrobacter ODSLC13 strain on the nitrate substrate comprises the following steps:

[0090] ①Culturable VBNC Arthrobacter ODSLC13 strains were preserved, and under aseptic conditions, a small amount of strains were picked and transferred to NYGR medium, and activated at 30°C for 2-4 days;

[0091] ②Pick a small amount of activated cultured bacteria ①Inoculate into PYGN liquid medium for pre-cultivation at 30°C for 2 days;

[0092] ③Introduce 2% of the pre-culture bacteria solution into the Giltay medium, culture on a shaker at 30°C and 120 rpm for 2 days, observe the gas production and color change of the medium, and measure ammonia nitrogen and nitrous in the culture medium nitrogen, nitrate nitrogen and total nitrogen.

[0093] Ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen were respectively used by Nessler colorimetry, ultraviolet...

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Abstract

The invention discloses a resurrecting culturable VBNC (viable but non-culturable) arthrobacterium ODSLC13 strain as well as a resurrecting method and application thereof. The resurrecting culturable VBNC arthrobacterium ODSLC13 (Arthrobacter sp.ODSC13) strain with the preservation number of CCTCC No:M2014074 is preserved in the China Center for Type Culture Collection located in Wuhan University on March 5, 2014. The strain has the beneficial effects that Giltay culture medium reaction liquid starts to turn dark blue from green and has a phenomenon of generating gas, which indicates that the strain can be reduced to form an alkaline substance by utilizing nitrate, and generates gas to change colors of an indicator; furthermore, the conversion rate of the measured nitrate nitrogen is 63.7 percent, the total nitrogen decrement rate is 57.9 percent, remaining of ammoniacal nitrogen and nitrite nitrogen cannot be detected, and a remarkable effect of nitrogen removal by denitrification can be achieved.

Description

technical field [0001] The present invention relates to a bacterial strain and its resuscitating method and application, more specifically, it relates to a resuscitating and cultivable VBNC Arthrobacter ODSLC13 strain and its resuscitating method and application. Background technique [0002] The biological treatment of ammonia nitrogen-containing sewage is an extremely important technical measure in today's agriculture and environmental protection industries. Among them, the oxidation of ammonia nitrogen, the oxidation of nitrite nitrogen, the reduction of nitrate nitrogen and the denitrification of key technical links need more With the participation of various microorganisms, it degrades and transforms inorganic and organic nitrogen. Generally, traditional separable single-function nitrosifying bacteria such as Nitrosomonas and Nitrosococcus species, nitrifying bacteria such as Nitrobacter and Nitrococcus species, denitrifying and denitrifying bacteria such as Micrococcus...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/38
Inventor 丁林贤周黎宏
Owner 丁林贤
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