Method for utilizing NaCl solution to separate nematode

A technology of nematodes and solutions, applied in the field of nematode separation, can solve the problems of nematodes' susceptibility to damage, heavy workload, and power consumption, and achieve the effects of reducing interference factors, reducing workload, and reducing working hours

Inactive Publication Date: 2012-07-25
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems that the existing sucrose centrifugal flotation method for separating nematodes takes a long time, the workload is large, the nematodes are easily damaged and consumes electric energy. The present invention provides a method for separating nematodes using NaCl solution. method

Method used

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specific Embodiment approach 1

[0010] Specific Embodiment 1: In this embodiment, a method for separating nematodes using NaCl solution is achieved through the following steps: 1. Weigh the nematode carrier sample, then add it to a NaCl solution with a concentration of 4-6 mol / L and stir for 5-30 minutes, Obtain a mixed solution, wherein the mass ratio of the NaCl solution to the nematode carrier sample is 5 to 20: 1; 2. The mixed solution obtained in step 1 is left to settle for 20 to 60 minutes to obtain supernatant and sediment; 3. 2. The obtained sediment replaces the nematode carrier sample in step 1, repeats the operation 2 to 6 times according to the method of step 1 and step 2, then discards the sediment, and obtains 3 to 7 parts together with the supernatant obtained in step 2 Supernatant; 4. Stack the 80 mesh and 500 mesh sampling sieves together, and place the 500 mesh sampling sieve under the 80 mesh sampling sieve to obtain a sieve group, and then use 3 to 7 parts of the supernatant obtained in s...

specific Embodiment approach 2

[0014] Embodiment 2: This embodiment differs from Embodiment 1 in that the concentration of the NaCl solution in Step 1 is 4.5-5.5 mol / L. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Embodiment 3: This embodiment differs from Embodiment 1 in that the concentration of the NaCl solution in step 1 is 5 mol / L. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for utilizing NaCl solution to separate nematode, which relates to a method for separating the nematode, and aims to solve the problems that: the used time is longer, the working amount is large, the nematode is easy to damage and the electric energy is consumed when an existing sucrose centrifugal flotation method is used for separating the nematode. The method for utilizing the NaCl solution to separate the nematode comprises the flowing steps of: I, adding a nematode carrier sample into the NaCl solution, and stirring to obtain mixed liquor; II, stewing the mixed liquor to obtain liquid supernatant and precipitates; III, replacing the nematode carrier sample with the precipitates, repeating the steps, removing the precipitates, and obtaining liquid supernatant; and IV, merging the liquid supernatant, injecting the liquid supernatant into a sample sieve group, and carrying out back flushing on a high-mesh sample sieve by utilizing water to obtain the nematode. The method can be applied to fields of biology, geography, environmental sciences and the like.

Description

technical field [0001] The invention relates to a nematode isolation method. Background technique [0002] Nematodes, also known as round worms, are the largest animal group on earth and are a general term for all worms. It can parasitize animals and plants, or live freely in soil, freshwater and seawater environments. Because of its wide distribution and diverse habitats, it has been widely studied in the fields of biology, geography and environmental science. The isolation of nematodes in nature is the most basic and crucial link in nematode research. Obtaining sufficient nematodes is the fundamental guarantee for research and application. At present, the separation methods of soil nematodes mainly use sucrose centrifugal flotation, Bellman funnel method and shallow dish method. [0003] Bellman funnel method and shallow plate method can only be used for the separation of live insects, and the separation efficiency is lower than that of sucrose centrifugal flotation. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
Inventor 王云彪闫修民武海涛吴东辉
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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