Pollution-free culture method for cordyce militaris

A technology of Cordyceps militaris and its cultivation method, which is applied in the field of pollution-free cultivation of Cordyceps militaris, and can solve the problems of few wild resources of Cordyceps militaris, limited sources of natural medicinal materials, and large dosage.

Inactive Publication Date: 2012-08-01
常熟市新港农产品产销有限公司
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  • Application Information

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Problems solved by technology

However, due to the very few wild resources of Cordyceps militaris, the source of natural medicinal materials is very limited, and the price is expensive, so the artificial cultivation of Cordyceps militaris has special significance.
However, with the increasing use of Cordyceps sinensis, the amount of use is increasing, resulting in the excessive collection of Cordyceps sinensis resources and the depletion of wild resources

Method used

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Embodiment Construction

[0005] The preferred embodiments of the present invention will be described in detail below; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.

[0006] Activation of strains: The slant strains stored in the refrigerator were activated by solid culture method or liquid fermentation method, and the activated strains were obtained by static culture at 25 °C for 48 hours; the solid culture method was prepared according to the formula of PDA comprehensive medium, and after high-pressure sterilization Prepare the slant, insert the preserved strains into the newly prepared medium, and culture the grafted strains in a 25°C incubator until the white mycelium covers the slant of the test tube; the liquid fermentation method uses the PDA slant medium The activated Cordyceps militaris was added to the above five liquid media at 25°C, 130 r min -1 and 12 h of light for ...

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Abstract

The invention discloses a pollution-free culture method for cordyce militaris. The pollution-free culture method comprises the following steps of: activating strains: activating and culturing slant strains stored in a refrigerator by using a solid culture method or a liquid fermentation method; and preparing a culture medium: filling 300g of rice, 20 go of coarse corn meal and 0.156g / g of silkworm pupa meal in each culture box, binding by using polypropylene films, sterilizing at 121DEG C for 1.5 hours, inoculating 100ml of liquid strain into each box to make the strain uniformly cover the surface of the culture medium, transferring the culture boxes to an incubator at the constant temperature of 25DEG C, transferring the culture boxes into a cordyce militaris growth chamber after mycelia are full of the culture boxes, making 4 to 6 holes in the center of each film, keeping the relative humidity of 58-95 percent and sufficient scattered light, keeping the temperature of 20DEG C, and harvesting when fruit bodies growth to the height of 5-8cm and the tops of the fruit bodies are full of granular asci.

Description

Technical field [0001] The present invention involves a method of non -rigorous cultivation in northern Cordyceps sinensis. Background technique [0002] Cordyceps Cordyceps, belongs to Ascomycotina, Clavicipitaceae, Cordyceps, also known as northern Cordyceps sinensis. It is a relatively famous food fungus in my country and the world. The effects of kidney, hemostatic and phlegm, inhibit tumors, delay aging, and improve the body's immunity.Cordyceps has attracted more and more attention to its good anti -tumor, antiviral, antibacterial anti -inflammatory, anti -fatigue, and enhancement of the body's immunity.A large number of pharmaceutical chemistry, physical and clinical studies have shown that the efficacy of Cordyceps soda is very similar to Cordyceps sinensis. Its main medicinal ingredients, Cordybenonic acid, Cordyceps polysaccharide content, and SOD activity are higher than Cordyceps sinensis. Thereforereplacement of.However, because there are very few wild resources for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 张祖学
Owner 常熟市新港农产品产销有限公司
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