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Artificial propagating method of semilabeo obscurus

A technology for artificial reproduction and dark lip fish, which is applied in fish farming, application, climate change adaptation and other directions, can solve the problems such as the same reports on the invention, and achieve the effects of low cost, simple operation and high hatching rate.

Active Publication Date: 2012-08-01
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Through document retrieval, do not see identical report with the present invention

Method used

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Embodiment Construction

[0012] At 18:00 on April 5, 2011, 6 groups of sexually mature broodstock were selected from the dark-colored lip fish collected from Lixianjiang domesticated in 2006, and injected according to the ratio of 1 female: 2 males. Artificial insemination was carried out at 16:00 on the 6th, and 24,000 fertilized eggs were obtained. The hatching was completed on April 11, and 22,800 fry were hatched, with a hatching rate as high as 95%. The specific method is as follows:

[0013] a. Wet fertilization: use of luteinizing hormone-releasing hormone analog (LHRH-A 2 ) + dioconone maleate (DOM) mixed oxytocic agent was successful in inducing labor in the dark lip fish. The dose is 1 mg dioconone maleate + 1 μg luteinizing hormone releasing hormone A per kg body weight 2 , the injection volume of male fish was halved. When the water temperature is 21°C, the effect time is 22 hours, and the artificial wet fertilization is carried out.

[0014] Artificial wet fertilization: Gently squeez...

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Abstract

The invention relates to an artificial propagating method of semilabeo obscurus and belongs to the technical field of fish product culture. According to the method, domperidone and lutein releasing hormone A2 are injected in gonadosomatic semilabeo obscurus according to a dosage that 1mg of domperidone and 1mug of lutein releasing hormone A2 are injected for each kilogram of body weight, the injection dosage is reduced to a half for milters, and the artificial wet process fertilization is carried out at the effect time of 22 hours and at the water temperature being 21 DEG C; ova and sperms of the gonadosomatic semilabeo obscurus are extruded into a container, 21-DEG C clean water is immediately poured into the container, and the water quantity is proper when the ova and sperms are immersed; then, the ova and sperms are slightly stirred for 30 seconds by chicken feather, and next, the incubation is prepared after the washing by clean water for three times; firstly, the fertilized ova are placed into 0.15ppm Tuzet water solution to be soaked for 15 minutes for sterilization and are then placed into large basins containing 60kg water, 3000 ova with the ovum diameter being 2.2 to 2.5 mm are placed into each basin, oxygenation is carried out through an oxygenation pump, the water temperature is 21 to 22 DEG C, the pH is 7.8 to 8.5, the incubation lasts 36 hours, the complicated reticular yolk system and the well developed somite and blood vessels can be clearly seen, and the semilabeo obscurus fry is obtained after 72-hour hatching. The artificial propagating method has the advantages that the cost is low, the operation is simple and convenient, the hatching rate is high, and the artificial propagating method can be put into production and practical use.

Description

Technical field: [0001] The invention relates to an artificial propagation method of dark lip fish, which belongs to the technical field of fish product cultivation. Background technique: [0002] Dark lip fish (Semilabeo obscures Lin) also known as pig mouth fish, horse nose hook. It belongs to the genus Semilabeo of the Cyprinidae subfamily Labeoninae. The dark lip fish is mainly distributed in the Nanpanjiang and Yuanjiang rivers of our country, and is unique to our country. Because of its fat body, tender meat and delicious taste, it has become the first choice for fishing. And because of its living habit of living in caves in groups, it has become the biggest victim of electricity, poison, and bombing by criminals. With the increase in the utilization of fish resources and the update of fishing technology, the biodiversity of fish is declining. Dark lips Fish has become a rare and endangered species in the Lixianjiang River Basin. In order to effectively protect the...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨君兴潘晓赋李再云陈小勇
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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