Method for producing EPA (Eicosapentaenoic Acid) by using eustigmatoa cf. polyphem

Abstract

The invention discloses a method for producing EPA (Eicosapentaenoic Acid) by using eustigmatoa cf. polyphem. The method comprises the following steps of: after gradual amplification culture of algae strains, calculating the amount of required algae liquid according to 20-30 percent of inoculation proportion; and transferring into an outdoor photobioreactor to perform large-scale raising when the optical density OD750 of the algae liquid reaches 2.5, wherein urea or nitrate is selected as a nitrogen source during the large-scale raising, the concentration of nitrogen is 2- 6 mM, the culture light intensity is controlled to be 100-600 uE / m<2>s, and the culture period is 8-12 days. Compared with the prior art, according to the method for producing the EPA by using the eustigmatoa cf. polyphem provided by the invention, high-efficiency production of the EPA can be realized.

Description

technical field [0001] The invention relates to the field of microalgae, in particular to a method for producing EPA by using Euophthalmos bordetii-like. Background technique [0002] Eicosapentaenoic acid (EPA, ω-3) is a polyunsaturated fatty acid, which has many biological activities and has attracted widespread attention. The earliest scholars who discovered that EPA is beneficial to human health were Bang and Dyerbery (1978) in Denmark. Subsequently, many scientists conducted in-depth research on the functions of EPA in nutrition and medicine, and found that EPA is a prostaglandin and its derivative prostacyclin. Precursors of hormone compounds such as , thrombin, and leukotrienes, have the functions of antithrombosis, lowering blood lipids, preventing platelet aggregation, and relaxing blood vessels, and have good prevention and treatment of human cardiovascular and cerebrovascular diseases, arthritis, nephritis, etc. In addition, EPA can also promote the growth and de...

AI Technical Summary

Problems solved by technology

[0006] 1) The quality of fish oil will be affected by the type of fish, fishing season and location; environmental pollution, non-purpose fatty acids and fishy smell will also affect the quality of fish oil; in addition, fish oil also has disadvantages such as high processing cost and easy oxidation. With the increasing shortage of fishery resources, it will be difficult for fish oil to meet people's market demand for EPA;

[0007] 2) There are few types of anaerobic microorganisms rich in EPA that have been isolated at present. The growth of anaerobic microorganisms requires a rich medium, and they are easily contaminated by bacteria during the cultivation process. Strict control of aseptic operations is required, which increases the cultivation cost. Newly discovered anaerobic microorganisms producing EPA require detailed safety evaluation;

[0008] Although some foreign companies use microalgae to produce EPA, the algae strains (Nitzschia alba) they use need to be cultured heterotrophically, and the cultivation process is complicated.

Images