Soft-shelled turtle systemic septicemia spherical virus inactivated vaccine and its preparation method
A spherovirus and inactivated vaccine technology, applied in the field of vaccines, can solve the problems of no preparation method for soft-shelled turtle systemic sepsis spherovirus inactivated vaccine, no prevention and treatment method, etc., and achieves easy operation and implementation, high protection rate and strong immunity. Effect
Inactive Publication Date: 2013-10-02
NINGBO UNIV
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Problems solved by technology
For soft-shelled turtle systemic septicemia, there are currently no researches on prevention and treatment methods at home and abroad, and there is no relevant research report on the inactivated vaccine of soft-shelled turtle systemic septicemia spheroid virus and its preparation method
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[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
1. Specific examples
[0025] The invention discloses an inactivated vaccine of soft-shelled turtle systemic septicemia spheroid virus, which contains the inactivated soft-shelled turtle systemic septicemia spheroid virus with the preservation number of CGMCC No.5378. The soft-shelled turtle systemic septicemia spherical virus (STSSSV), the strain is P1015 strain, the preservation number is CGMCC No.5378, and it was preserved in the China Microorganism Culture Collection Management Committee on October 21, 2011 General Microbiology Center. The virus has no envelope, 23-40nm in diameter, and can proliferate both inside and outside the nucleus. The specific preparation method is as follows:
[0026] (1) Virus purification
[0027] Take the soft-shelled turtle with systemic sepsis, cut the visceral tissue of the diseased soft-shelled turtle in...
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Abstract
The invention discloses a soft-shelled turtle systemic septicemia spherical virus inactivated vaccine and its preparation method, and is characterized in that the vaccine contains the inactivated soft-shelled turtle systemic septicemia spherical virus with the collection number being CGMCCNo.5378. The preparation method comprises the following steps of: cutting internal organ of sick soft-shelled turtles into pieces, adding into a TNE buffer, centrifuging, taking a supernatant, centrifuging, taking a white precipitate, suspending in the TNE buffer, centrifuging the precipitate, taking the supernatant, and centrifuging to obtain a white precipitate, namely the purified virus; diluting the purified virus by the use of aseptic normal saline until the final concentration of viral protein reaches 0.5-1mg / ml, and adding 0.5% (final volume) of formalin for inactivation in a thermostat water bath cauldron; and completely emulsifying inactivated virus and a complete Freund's adjuvant according to the volume ratio of 1:1 to obtain the inactivated vaccine. The soft-shelled turtle systemic septicemia spherical virus inactivated vaccine proposed for the first time is safe and effective and has strong immunity, and the preparation method is simple and easy to operate.
Description
technical field [0001] The invention relates to the technical field of vaccines, in particular to an inactivated soft-shelled turtle systemic sepsis globular virus vaccine and a preparation method thereof. Background technique [0002] Soft-shelled turtle, scientific name Chinese soft-shelled turtle, has always been a medicinal food and nourishing good product. In the 1980s, the rise of greenhouse farming greatly increased the production of soft-shelled turtles, which were originally considered rare, followed by the wanton prevalence of diseases. Antibiotics and chemicals are often used for traditional prevention and control. Instead of treating viral diseases, they have poisoned soft-shelled turtles, polluted the environment, and caused drug residues, which undermined consumer confidence. Since 2007, a kind of systemic sepsis has broken out in the Zhejiang area, especially the soft-shelled turtle ecological farms in Hangzhou and Ningbo. Through studies such as artificial ...
Claims
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IPC IPC(8): A61K39/12A61K9/107A61P31/12C12N7/02C12N7/06
Inventor 李登峰周永强刘联国彭娇
Owner NINGBO UNIV