Chicken embryo culture method of Portunus tritubereulatus reovirus

Abstract

The invention discloses a chicken embryo culture method of Portunus tritubereulatus reovirus and is characterized by comprising the following steps of: using SPF chicken embryo as an in vitro culture system of Portunus tritubereulatus reovirus, selecting healthy chicken embryo of 3 and 4 days-old, inoculating a prepared Portunus tritubereulatus reovirus inoculated solution onto a chorioallantoic membrane of the chicken embryo, continuously culturing until the chicken embryo is dead, collecting dead chicken embryo cultured for 3-10 days, separating the chorioallantoic membrane and amniotic fluid, mixing the chorioallantoic membrane and the amniotic fluid, homogenizing the mixture, centrifuging and filtering to obtain a supernatant of Portunus tritubereulatus reovirus. The invention has the following advantages: a more efficient and adaptive Portunus tritubereulatus reovirus in vitro culture method is provided for rapid and effective in vitro subcultring of the virus; and the method overcomes the defect that there is no adaptive culture system during the culture process of Portunus tritubereulatus reovirus, fills the blank of chicken embryo culture system of crab virus and has laid a foundation for further research and application.

Description

Technical field [0001] The invention relates to an in vitro culture method of Portunus tritubercula reovirus, in particular to a chicken embryo culturing method of Portunus tritubercula reovirus. Background technique [0002] Reoviruses are viruses with a wide range of hosts, and their hosts can include mammals, birds, insects, and aquatic animals. A type of reovirus that infects aquatic organisms is classified as aquatic reovirus. Meyers is equivalent to the first aquatic reovirus isolated from American oysters in 1979. At present, there are more than 40 aquatic reoviruses isolated and identified. These viruses infect the host mainly through respiration and intestines, causing host hemorrhage and liver and pancreas diseases. It has caused huge losses in the global aquaculture industry and seriously threatened the development of the aquaculture industry. Aquatic reovirus is a ubiquitous virus in aquatic animals and has been reported in fish, shrimp, and crabs. [0003] Portunu...

AI Technical Summary

Problems solved by technology

However, virus chicken embryo culture is more common in the cultivation of some higher animal viruses, and its research and application in the field of aquatic animal viruses is extremely rare

At present, there is no relevant research report on the method of cultivating crab viruses including portunus trituberculatus reovirus by using chicken embryo culture method at home and abroad

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