Application of functionalized nano-selenium in inhibiting tumor angiogenesis and antitumor drugs
A technology for tumor angiogenesis and anti-tumor drugs, applied in anti-tumor drugs, drug combinations, active ingredients of sulfur/selenium/tellurium, etc., can solve the problems of undiscovered nano-selenium, increase drug intake, and improve targeting , The effect of simple product system
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Embodiment 1
[0042] The preparation of the nano selenium of embodiment 1 heteropolyacid-transferrin modification
[0043] (1) Weigh 1.5g of dodecatungstophosphoric acid and dissolve it in 2mL of distilled water to prepare a 0.5mol / L storage solution, adjust the pH to 3.5~5.5 with 1mol / L HAc-NaAc buffer solution, and set the temperature in N 2 Under the atmospheric atmosphere, use a potentiostat to control potential electrolytic reduction, and the degree of reduction is indicated by a copper coulomb meter. When the electrolysis reaches two-electron reduction, the electrolysis is stopped, and the electrolyte is placed in a vacuum desiccator to precipitate crystals. The crystals were recrystallized 2 to 3 times to obtain the treated heteropolycyanate compound.
[0044] (2) Prepare dodecatungstophosphate heteropoly blue into a 0.5mmol / L solution, add it dropwise to 0.5-1mmol / L sodium selenite solution and mix evenly under continuous stirring, and finally dodecatungsten The molar ratio of hete...
Embodiment 2
[0046] The preparation of the nano selenium of embodiment 2 gallic acid-transferrin modification
[0047] Na 2 SeO 3 Prepare a 5mmol / L solution with distilled water, prepare a 5mmol / L solution of gallic acid with distilled water, add the gallic acid solution dropwise to the sodium selenite solution under constant stirring, and adjust the pH with 0.1mol / L hydrochloric acid solution 2.8-3.5, heated in a 70°C water bath until the solvent volatilized and dried to obtain an orange-red nano-selenium sample.
[0048] (2) get above-mentioned nano selenium and be mixed with 0.5mmol / mL solution, adjust its pH between 2.3~5.0 with the hydrochloric acid solution of 0.1mmol / mL, transferrin is mixed with the solution of 0.2mmol / mL with triethanolamine, in Add it dropwise to the above-mentioned nano-selenium solution under constant stirring, keep the pH of the solution between 2.3 and 5.0 during the process, continue to stir for 12 to 24 hours, place it at 4°C for 12 hours, centrifuge, was...
Embodiment 3
[0049] Embodiment 3 as the in vitro experiment of antitumor drug
[0050] In this experiment, a variety of tumor cell lines purchased from ATCC were selected, including human malignant melanoma cells (A375), human liver cancer cells (HepG2), human colon cancer cells (SW480), human prostate cancer (PC-3), and human breast cancer cells. cells (MCF-7), human lung adenocarcinoma cells (A549). The sample numbers tested are: a (nano-selenium modified by dodecatungstophosphoric acid-transferrin), b (nano-selenium sol modified by dodecatungstophosphoric acid), c (nano-selenium modified by gallic acid-transferrin), d (gallic acid modified nano-selenium sol) and cisplatin.
[0051] (1) MTT test method
[0052] Take tumor cells in the logarithmic growth phase and adjust the concentration of viable cells to 2×10 4 / ml was added to a 96-well culture plate, 100 μl per well, cultured in an incubator for 24 hours, and then added 100 μl of test samples with different concentrations. The neg...
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