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Sheep K71 skin hair follicle specificity promoter and clone thereof

A technology of skin hair follicles and promoters, applied in the field of molecular genetics

Active Publication Date: 2014-12-10
新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research report on sheep, and the reporter sequence of this gene only has a partial sequence of the coding region

Method used

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  • Sheep K71 skin hair follicle specificity promoter and clone thereof
  • Sheep K71 skin hair follicle specificity promoter and clone thereof
  • Sheep K71 skin hair follicle specificity promoter and clone thereof

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Experimental program
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Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0015] The invention relates to the genetic resources of Xinjiang fine-wool sheep, which are self-collected at the sheep breeding base of Xinjiang Academy of Animal Husbandry Sciences in Urumqi, Xinjiang, China.

[0016] 1. Cloning of the 5' regulatory region of sheep K71 gene

[0017] (1) Sheep blood was collected, anticoagulated with sodium heparin, and genomic DNA was extracted by phenol-chloroform extraction.

[0018] (2) Using the Genome Walking Kit (Dalian Bao Biology) AP3 combined with three downstream primers designed to amplify the 5' regulatory region of the sheep K71 gene, the primer sequences are shown in Table 1, please refer to the sequence table SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4. According to the reaction system and reaction procedure provided in the kit, the results are shown in figure 1 , figure 2 .

[00...

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Abstract

The invention discloses a sheep K71 skin hair follicle specificity promoter and clone thereof, i.e. a promoter of keratin 71 (K71), wherein the nucleotide sequence of the promoter is expressed by a sequence table SEQ ID NO:1. The invention provides the clone of the promoter in a sheep skin hair follicle specificity expression K71 gene 5' regulating and controlling region, and verifies the characteristic of the promoter by reporting gene analysis. According to the invention, the sequence of the cloned K71 gene 5' promoter regulating and controlling region only specially starts the transcription expression of the downstream objective gene of the promoter in the skin hair follicle, so that the requirement of the gene to the specificity expression of the skin hair follicle can be met, and the invention can be applicable to the cultivation of the transgenosis sheep.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to a sheep skin hair follicle-specific promoter and its clone, in particular to the cloning analysis of a skin hair follicle-specific expression gene keratin 71 (K71) 5' regulatory region-specific promoter. Background technique [0002] Gene expression and gene function are not only the core content of molecular biology research (the concept of gene here is not just the DNA sequence encoding protein), but also a key link in the application of molecular biology techniques. Transcription regulation plays an important role in gene expression, and its regulation mechanism is also very complex. Among them, the promoter interacts with the transcription factors that regulate the initiation of transcription, strictly controls the initiation time, location and expression abundance of gene expression (transcription), and determines the tissue specificity and expression intensity of gene transcript...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/85A01K67/027
Inventor 李文蓉贺三刚张雪梅张宁玛依拉刘明军
Owner 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心