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SNP molecular marker easy to domesticate artificial feed mandarin fish related gene

A technology of molecular markers and artificial feed, applied in the direction of recombinant DNA technology, DNA / RNA fragments, microbial measurement / inspection, etc., can solve the problems that have not yet seen the report of SNP molecular markers

Inactive Publication Date: 2012-09-05
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0011] So far, there have been no reports on SNP molecular markers of genes related to domestication of mandarin fish on artificial diet

Method used

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  • SNP molecular marker easy to domesticate artificial feed mandarin fish related gene
  • SNP molecular marker easy to domesticate artificial feed mandarin fish related gene
  • SNP molecular marker easy to domesticate artificial feed mandarin fish related gene

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Effect test

Embodiment 1

[0036] 1.1.1 Test animals

[0037] The mandarin fish seedlings used were bred and raised to a size of 5-6 cm from the Xinrong Fry Breeding Farm in Foshan City, Guangdong Province, and then fed and domesticated in the Freshwater Seedling Breeding Center of Guangdong Province. The method of domestication is: first feed with fresh and alive small fish for 3 days, starve for 2 days; then feed with half-dead small fish for 3 days; feed with whole dead small fish for 3 days; In the two groups of domestication, before sampling, the mandarin fish of the easy domestication group and the difficult domestication group were fed with fresh small fish for 3 days, and samples were taken after 2 days of starvation. After two weeks of feeding and acclimation, two groups of mandarin mandarin juveniles were selected from 1200 feeding fry, which were easy to domesticate and difficult to domesticate, with 120 fish in each group.

[0038] 1.2 Method

[0039] 1.2.1 Genomic DNA extraction of mandar...

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Abstract

The invention belongs to the technical field of fish breeding, relates to the technical field of molecular markers, and particularly relates to an SNP molecular marker used for detecting and domesticating artificial feed mandarin fish related gene polymorphism. The SNP molecular marker is characterized in that the molecular marker is a nucleotide sequence corresponding to the following gene fragments: (1) the SNP molecular marker of a neuropeptide Y gene, with the nucleotide sequence as shown in sequence table SEQ IDNO: 1; (2) the SNP molecular marker of a pepsin gene, with the nucleotide sequence as shown in sequence table SEQ ID NO: 2; and (3) the SNP molecular marker of a lipoprotein lipase gene, with the nucleotide sequence as shown in sequence table SEQ ID NO: 3. The invention relates to the SNP molecular marker for domesticating artificial feed mandarin fish related gene polymorphism of mandarin fishes,and can be used for marker-assisted selection of the mandarin fishes.

Description

technical field [0001] The invention belongs to the technical field of preparation of fish molecular markers, and in particular relates to the preparation of a SNP molecular marker of a gene related to mandarin fish that is easy to tame and eat artificial feed. The molecular marker can be applied to auxiliary selection of mandarin fish breeding genotypes. Background technique [0002] Mandarin fish (Siniperca chuatsi), also known as mandarin fish (Siniperca chuatsi), is a rare freshwater fish belonging to the suborder Perciformes. Due to its fast growth and other characteristics, the cocked mandarin fish has become the main species in Chinese mandarin fish culture. However, this fish has a peculiar food habit, and it feeds on live fish and shrimps all its life. Usually, it absolutely refuses to eat dead bait or compound feed. The phenomenon is very rare in fish [1] . The commercial culture of mandarin fish has always been carried out by feeding live bait, and mandarin fish...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 梁旭方何珊杨宇晖方荣曹亮杜嵇华何焱符云叶卫
Owner HUAZHONG AGRI UNIV
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