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New bacterial strain of pholiota squarrosoides and sporocarp culture method thereof

A cultivation method and fruiting body technology, which are applied in the field of new strains of C. chinensis and their fruiting bodies, can solve the problems of few and no domesticated cultivation of C. The effect of short time and fast speed when the silk bag is full

Active Publication Date: 2013-08-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literatures on C. chinensis, and there is no report on domestication and cultivation.

Method used

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  • New bacterial strain of pholiota squarrosoides and sporocarp culture method thereof
  • New bacterial strain of pholiota squarrosoides and sporocarp culture method thereof
  • New bacterial strain of pholiota squarrosoides and sporocarp culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Isolation and Identification of Pholiota squarrosoides HS4

[0043]The wild fruiting body of the strain of the present invention (referred to as HS4) is collected from a persimmon tree in Changping, Beijing, and identified as Pholiota squarrosoides by Mr. Mao Xiaolan, Institute of Microbiology, Chinese Academy of Sciences. Further, through the observation of the morphological characteristics of its fruiting body and mycelium, and the sequencing of the intergenic sequence (ITS), the biological taxonomy of the strain was determined.

[0044] 1. Morphological characteristics

[0045] 1. Morphological characteristics of fruiting bodies

[0046] Bacterial strain HS4 fruiting bodies of the present invention are generally medium and large ( figure 1 ). Cap diameter 3-12cm, hemispherical to oblate hemispherical, finally flat, sometimes slightly convex in the middle, dry surface, yellowish brown or earthy brown with pink, scales pyramid-like, spiny, darker in color...

Embodiment 2

[0052] Example 2. Artificial cultivation of fruiting bodies of Pholiota squarrosoides HS4CGMCC No.6062

[0053] 1. Preparation of strains

[0054] (1) Mother seed preparation: inoculate the Pholiota squarrosoides HS4CGMCC No.6062 obtained in Example 1 on the mother seed medium, and culture it in the dark at 24°C-28°C for 7-10d to obtain The mycelium is used as the mother seed; if the obtained mother seed is not used in time, it can be stored at 4°C.

[0055] (2) Original seed preparation: transfer the mother seed obtained in step (1) to the original seed medium, cultivate at a temperature of 23°C-25°C, and a relative air humidity of 50%-60%, well ventilated and protected from light Until the mycelia cover the container, the original species is obtained. The storage period of the original species at room temperature generally does not exceed 15 days, and the storage period at low temperature does not exceed 20 days. Expired strains cannot be used.

[0056] (3) Preparation of...

Embodiment 3

[0065] Example 3. Detection of Mycelia Growth Rate, Yield and Nutrient Components of Pholiota squarrosoides HS4CGMCC No.6062

[0066] In this example, Pholiota adipose No. 1 was used as a control (currently there is no report on the cultivation of Pholiota squarrosoides, and only its close relative species Pholiota squarrosoides can be used as a control) to detect Pholiota squarrosoides Mycelium growth rate, yield and nutrient composition of HS4CGMCC No.6062.

[0067] 1. Determination of mycelium growth rate

[0068] 1. Experimental materials

[0069] The mother species, original species and cultivars of Pholiota squarrosoides HS4CGMCC No.6062 and Pholiota adipose No. 1 strain, the preparation method of which is the same as step 1 of Example 2.

[0070] 2. Experimental method

[0071] Determination of the average growth rate of the mycelium of the mother species: the mother species of the two strains were inoculated in the center of the medium surface of the mother species,...

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Abstract

The invention discloses a new bacterial strain of pholiota squarrosoides and a sporocarp culture method thereof. The pholiota squarrosoides provided by the invention is specifically pholiota squarrosoides HS4 which has a preservation number of CGMCC No.6062 in the China General Microbiological Culture Collection Center (CGMCC). The hypha of the pholiota sguarrosoides HS4 with the preservation number of CGMCC No.6062 provided by the invention has high growth speed and short sackful time (which is advanced by 10d to 12d compared with that of pholiota adipose No.1), and can be taken as a hybridized parent to breed a new strain with high character hyphae breeding speed, short period and high yield. In addition, the pholiota squarrosoides HS4 with the preservation number of CGMCC No.6062 has high yield, and the biology efficiency of the first two tides of mushrooms reaches 51.29% which is higher than the biology efficiency of 42.67% of the pholiota adipose No.1.

Description

technical field [0001] The invention relates to a new bacterial strain of Umberella chinensis and a method for cultivating the fruiting bodies thereof. Background technique [0002] Phoriota squarrosoides (Peck) Sacc scientific name Pholiota squarrosoides (Peck) Sacc. In taxonomy, it belongs to the kingdom of fungi, basidiomycota, layer fungi, agarics, strophariaceae, and ring rust umbrella. The fruiting body of the apical scale ring rust umbrella is bright and golden yellow, and the stipe of the cap is covered with pyramid-shaped scales. The fungus is a medium-low temperature type edible fungus, which occurs in large quantities on tree stumps and fallen logs in the forest in summer and autumn, and has a high yield; its fruiting bodies are rich in protein, carbohydrates, vitamins and various mineral elements, and are delicious and unique in flavor. to be eaten by people. The morphological characteristics of Strophariae and Stropharia genus belong to Strophariaceae and Str...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H15/00A01G1/04C05G3/00
Inventor 王守现刘宇许峰王兰青赵爽耿小丽孟莉莉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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