Geranium strictipes R. Knuth effective part extract as well as preparation method and drug application

An effective part, the technology of purple burnet, applied in the field of medicine, to achieve the effect of less pollution, better effect and less loss of samples

Inactive Publication Date: 2012-09-19
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Except that the inventor of this patent has conducted a preliminary study on the antibacterial effect of its crude extract in th

Method used

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  • Geranium strictipes R. Knuth effective part extract as well as preparation method and drug application
  • Geranium strictipes R. Knuth effective part extract as well as preparation method and drug application
  • Geranium strictipes R. Knuth effective part extract as well as preparation method and drug application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of extracts from the effective parts of Ulmus pumila (50% EtOH)

[0026] 1.1 Instruments and reagents:

[0027] The freeze dryer (FDU-2100) is a product of Japan's EYELA Tokyo Physical and Chemical Equipment Co., Ltd. The ultra-clean workbench adopts the SW-CJ-2FD double single-sided purification workbench of Suzhou Purification Equipment Co., Ltd., and the water-proof constant temperature incubator is a Shanghai No. The product of Heng Technology Co., Ltd., the sterilizer is used by Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory YXQ-LS-50SII vertical pressure steam sterilizer, and the rotary evaporator (R-210) is a product of Swiss Buqi. The UV spectrum was measured with Shimadzu UV-240 UV spectrophotometer, the thin-layer preparative chromatography (PTLC) used Merck’s aluminum foil silica gel plate, the thin plate (TLC) detection used 254nm and 365nm UV lamps; the developer used iodine vapor, 10 % Sulfuric acid-ethanol and phosphomoly...

Embodiment 2

[0036] Example 2: Preparation of extracts from the effective parts of Ulmus pumila (90% EtOH)

[0037] 2.1 Apparatus and reagents: the same as in Example 1.

[0038] 2.2 Medicinal materials: the same as in Example 1.

[0039] 2.3 Preparation of the ethanol extract of Ulmus pumila

[0040] Take 5 kg of Geshanxiao medicinal material (dried root of Ulmus pumila), soak in 10000 ml of 85% ethanol, heat to 85°C for extraction, and extract 3 times for 2 hours each time. The extracts were combined and concentrated under reduced pressure to obtain 2.03 kilograms of ethanol extract of Ulmus pumila (sample number: ethanol extract B).

[0041] 2.4 Preparation of Ethyl Acetate Extract of Ulmus purpurea:

[0042] Take 500 g of ethanol extract B, extract a few times with ethyl acetate as a solvent, a total of 3 times, combine the extracts and concentrate under reduced pressure to obtain 88 g of ethyl acetate extract.

[0043] 2.5 Preparation of extracts from the effective parts of Ulmus pumila:

[0044...

Embodiment 3

[0045] Example 3: Test tube method to test the antibacterial effect of extracts from the effective parts of Ulmus pumila and various antibiotics on MRSA

[0046] 3.1 Screening of resistant strains:

[0047] Determination of methicillin-resistant Staphylococcus aureus (MRSA): First, use conventional methods to culture and isolate bacteria, obtain pure culture and then use VITEK automatic microbial analyzer to identify Staphylococcus aureus, and then according to the United States Clinical Laboratory Standardization Committee in 2004 Bacterial Drug Sensitivity Test Judgment Standard (NCCLS Standard, 2004) promulgated in 2004, using the trace broth method to determine the MIC value of isolated Staphylococcus aureus with oxacillin, the result of the determination is that the oxacillin MIC ≥ 0.4 μg / ml MRSA strain.

[0048] 3.2 Reagents and test drugs:

[0049] Mueller-Hinton agar medium (MH agar) (Hangzhou Tianhe Microbial Reagent Co., Ltd., batch number 070731), bacterial dry powder med...

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Abstract

The utility model relates to a geranium strictipes R. Knuth effective part extract as well as a preparation method and a drug application thereof. The geranium strictipes effective part extract is formed by extracting geranium strictipes R. Knuth belonging to a plant of geranium eriostemon with alcohol water, extracting with ethyl acetate, performing macroreticular resin column chromatography and eluting with alcohols solvent. When being combined with such antibiotics as penicillin, erythrocin, oxacillin, gentamicin, levofloxacin and ampicillin-sulbactam, the geranium strictipes R. Knuth effective part extract can effectively restrain the growth of methicillin-resistant staphylococcus aureus, extended-spectrum Beta-lactamases-producing Escherichia coli and other drug-resistant strains, has obvious efficacy of resisting drug-resistant strains with antibiotics synergistically, and can be used for preparing anti drug-resistant strain drugs combined with the antibiotics or health care products.

Description

Technical field [0001] The present invention relates to the technical field of medicine. In particular, the present invention relates to an extract of the effective part of Ulmus pumila and its preparation method and pharmaceutical use. The effective part extract is prepared from the Yunnan folk medicine Geshanxiao through a modern extraction process, has an obvious effect of synergistically resisting drug-resistant strains with antibiotics, and can be used to prepare anti-drug-resistant bacteria drugs or health products combined with antibiotics. Background technique [0002] In 2004, the drug-resistant, highly pathogenic Clostridium difficile was prevalent in North America and Western Europe. The outbreak in Quebec, Canada alone caused the deaths of 7,000 severely ill patients and 1,300 people. [0003] The Director of the World Health Organization (WHO) said: We are on the verge of a global crisis of infectious diseases, and no country is immune. According to the WHO report, o...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K45/00A61P31/04
CPCY02A50/30
Inventor 李辉洪小凤耿玲张娴文巫秀美施贵荣李凤贤
Owner DALI UNIV
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