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Fungus promoting germination of plant seed of Dendrobium of Orchidaceae

A technology of plant seeds and fungi, applied in production, can solve the problem that wild dendrobium resources cannot meet the demand and other problems

Inactive Publication Date: 2012-09-19
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, the demand for Dendrobium has increased greatly, especially with the advent of Chinese patent medicines such as Dendrobium Yeguang Pills and Mailuoning Injection, wild Dendrobium resources can no longer meet the demand
However, the main problem with this propagation method is that the seedlings formed after germination are relatively fragile, and there are many difficulties that cannot be overcome when transplanted from tissue culture conditions to field environments to continue growing.

Method used

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  • Fungus promoting germination of plant seed of Dendrobium of Orchidaceae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Effect of pw-3 on the germination of Dendrobium candidum seeds

[0011] Sow the seeds of Dendrobium officinale on the oat culture medium that has been covered with pw-3 fungal hyphae. Take the seeds sown on the sterile oat culture medium as the control, and cultivate in the culture room at 23-27℃. Investigate the germination of seeds at the end of the month. See Table 1 for the classification criteria for seed development levels of orchids. The results were as follows: the germination rate of D. officinale seeds inoculated with pw-3 fungus was 69.3%, and that of D. officinale seeds without fungus was 24.6%; and the seed germination rate of Dendrobium officinale inoculated with pw-3 fungi reached level 4, that is, leaves were differentiated. , The control group only developed to the second level, that is, the seeds expanded and broke, and no further development was seen.

[0012] Table 1 Grading standard for seed development grade of orchid family

[0013]

Embodiment 2

[0015] Effect of pw-3 on seed germination of Dendrobium nobile

[0016] Sow the seeds of Dendrobium nobile on the oat culture medium that has been covered with pw-3 fungal hyphae. Take the seeds sown on the sterile oat culture medium as the control, and cultivate in the culture room at 23-27℃. Investigate the germination of seeds at three months. The results are: the seed germination rate of Dendrobium nobile inoculated with pw-3 fungus is 56.6%, and the seed germination rate of Dendrobium nobile inoculated with fungus is 31.7%; and the germination rate of Dendrobium nobile inoculated with pw-3 fungus reaches level 5, namely One or two leaves grew and developed into seedlings. The control group only developed to the second level, that is, the seeds swelled and broke, and no further development was seen.

Embodiment 3

[0018] Effect of pw-3 on the germination of Dendrobium copper-bark seeds

[0019] Sow the seeds of Dendrobium copper-bark on the oat culture medium that has been covered with pw-3 fungal hyphae. Take the seeds sown on the sterile oat culture medium as the control, and cultivate them in the culture room at 23-27℃. Investigate the germination of the seeds at two months. The results were as follows: the germination rate of Dendrobium copper-bark seeds inoculated with pw-3 fungus was 92.0%, and the germination rate of Dendrobium copper-bark seeds without fungus was 54.3%; and the germination rate of Dendrobium copper-bark seeds inoculated with pw-3 fungus reached level 5, namely One or two leaves grew and developed into seedlings. The control group only developed to the second level, that is, the seeds swelled and broke, and no further development was seen.

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Abstract

The invention discloses shh-53 (Sebacina sp.) promoting germination of plant seed of Dendrobium of Orchidaceae. The shh-53 has important application value on sexual reproduction of plant of Dendrobium of Orchidaceae, large-scale production of seedlings and sustainable utilization of resources.

Description

Technical field [0001] The present invention relates to the technical field of bioengineering, in particular to two fungi capable of promoting seed germination of orchids of Dendrobium, used for the reproduction and production of Dendrobium plants, and protecting plant resources of the genus. Background technique [0002] Orchidaceae (Orchidaceae) is one of the large families of angiosperms. There are about 700 genera and nearly 20,000 species in the world, many of which are rare and endangered plants. All wild orchids are included in the protection scope of the "Convention on International Trade in Endangered Species of Wild Fauna and Flora", accounting for more than 90% of the plants to be protected by the convention, and are the "flagship" group in plant protection. Dendrobium is the second largest genus of Orchidaceae, many of which have high medicinal and ornamental value. According to the 2005 edition of "The Pharmacopoeia of the People's Republic of China": Dendrobium nob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/00C12R1/645
Inventor 郭顺星王卉陈娟陈晓梅孟志霞
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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