Detection method of JC virus as well as kit and application thereof

A detection kit, JC virus technology, applied in the determination/inspection of microorganisms, methods based on microorganisms, microorganisms, etc., can solve the problems of no detection methods or reagents, and achieve timely case diagnosis and treatment and treatment effect monitoring, effective and accurate Quantitative detection, good specificity
CN102690895BInactive Publication Date: 2013-05-01THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
Publication Date
2013-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a detection method of a virus and a kit and particularly relates to a detection method of a JC virus and a kit thereof. The detection comprises the following steps of: firstly, designing specific primers JCV-F and JCV-R and a Taqman fluorescence probe JCV-P aiming at a JC virus gene group; marking a fluorophore on a 5' end of the Taqman fluorescence probe JCV-P and marking a quencher at any position of the Taqman fluorescence probe JCV-P except the 5' end; then treating a sample to be detected and carrying out PCR (Polymerase Chain Reaction); and finally, detecting a reaction result by using a fluorescent quantitation PCR instrument. The detection method disclosed by the invention has the advantages that carrying out qualitative and quantitative detection can be realized, and high sensibility, good specificity, rapid reaction and low cost can be realized. The invention further relates to a detection kit of the JC virus and an application of the method.
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Description

technical field

[0001] The invention relates to a virus detection method and a kit, in particular to a JC virus detection method and a kit. Background technique

[0002] JC virus (JCV) is a human polyomavirus, a small double-stranded DNA virus. JCV was first discovered and isolated from patients with progressive multifocal leukoencephalopathy (Progressive Multifocal Lukoenphalapathy, PML) by PADGETT in 1971. The virus has only one serotype, but can be divided into more than 30 genotypes.

[0003] JCV is an opportunistic infectious pathogen, and the serological positive rate in the normal population is as high as 80%. Primary infection generally occurs in childhood and is mostly asymptomatic. JCV can be transmitted from mother to child through childbirth (placenta), breast-feeding or long-term co-living contact, and can also be transmitted through the respiratory and digestive tracts. After the initial infection, JCV exists in human tissues in a latent state, but when the...

Claims

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