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Preparation method of animal model simulating human pelvic inflammatory disease (PID)

An animal model and pelvic inflammatory disease technology, which is applied in the field of making an animal model of simulated human pelvic inflammatory disease, can solve problems such as hindering the application of the model and difficult operations, and achieve the effects of easy to master the method, easy operation method, and easy to obtain materials.

Inactive Publication Date: 2012-10-03
唐和斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The above two methods are different from the occurrence of clinical pelvic inflammatory disease, and the operation is more difficult, which hinders the application of the model

Method used

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  • Preparation method of animal model simulating human pelvic inflammatory disease (PID)
  • Preparation method of animal model simulating human pelvic inflammatory disease (PID)
  • Preparation method of animal model simulating human pelvic inflammatory disease (PID)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]Strain preparation: Escherichia coli strain (GIM1.457) was selected, inoculated in beef extract agar medium, and cultured at 37°C for 2 days.

[0042] Animal preparation: Wistar rats without reproductive tract diseases were selected, and 10 animals were selected for each strain.

[0043] Animal inoculation: inject Escherichia coli strain (GIM1.457) (100 μl, 1 × 10 8 CFU / ml) was inoculated into the vagina of rats for 5 weeks, twice a week.

[0044] Post-inoculation treatment and observation: 5 weeks after the first inoculation, kill the animal, and remove the reproductive organs, such as: vagina, uterus, fallopian tube, ovary, fully rinsed in sterile saline, fixed for 48 hours and then used for disease Physical examination.

[0045] Through observation and analysis, it is found that this method has the following advantages:

[0046] 1. The success rate of the model is as high as 90%. On the third day after vaccination, the genitals are found to be red and swollen, wit...

Embodiment 2

[0050] SD rats without reproductive tract diseases were selected, and the rats were treated with estradiol for 1 week before inoculation, 3 times a week. Select the Escherichia coli strain (GIM1.457), inoculate it in the beef extract agar medium, and cultivate it at 37°C for 3 days; inject the Escherichia coli strain (GIM1.457) (100 μl , 1 x 10 6 CFU / ml) was inoculated into the vagina of rats for 4 weeks, twice a week. Four weeks after the first inoculation, the rats were killed, and the reproductive organs, such as: vagina, uterus, fallopian tube, and ovary, were fully rinsed in sterile saline, fixed for 48 hours, and used for pathological examination.

[0051] The results showed: 1. The success rate of the model reached 100%. On the third day after inoculation, the genitals were found to be red and swollen, and the secretions increased (such as figure 1 . Coli A). After modeling, it was found through anatomy that the entire pelvic cavity showed an upward development of i...

Embodiment 3

[0054] SD rats without reproductive tract diseases were selected, and the rats were treated with estradiol for 1 week before inoculation, 3 times a week. Select the Escherichia coli strain (ATCC25922), inoculate it in the beef extract agar medium, and cultivate it at 37°C for 3 days; inject the Escherichia coli strain (ATCC25922) (100 μl, 1 × 10 7 CFU / ml) was inoculated into the vagina of rats for 5 weeks, 3 times a week. At the same time, continue to use estradiol treatment for 5 weeks, 3 times a week. Six weeks after the first inoculation, the rats were sacrificed, and the reproductive organs, such as: vagina, uterus, oviduct, and ovary, were fully rinsed in sterile saline, fixed for 48 hours, and used for pathological examination.

[0055] The results showed: 1. The success rate of the model reached 80%. On the 3rd day after the inoculation, the genitals were found to be red and swollen, and the secretions increased. After modeling, it was found through anatomy that the ...

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Abstract

The invention relates to a preparation method of animal model simulating human pelvic inflammatory disease (PID). The method comprises the steps of selecting animal of family Muridae without reproductive tract diseases; and under sterile condition, inoculating strain of genus Enterobacter, genus Staphylococcus or genus Candida in animal vagina for 1-8 weeks and 1-7 times every week. The invention has the advantages of high model preparation success ratio and no dependence on immunity inhibitor. After success of model preparation, vaginal secretion obviously increases; bacteria can be observed by vaginal smear microscopic examination; metryperemia, abscess and ovarian cyst can be observed after dissection. Large amount of inflammatory cell infiltration can be observed in uterus and oviduct by organ tissue section microscopic examination. Blood examination indicates abnormal rise of neutrophil count. The method has the advantages of simple implementation, easily-accessible materials, easy grasping without special technical requirements for operator, accordance with clinical natural occurrence process of PID, and suitability for research of generation, development and mechanism of PID and screening of relevant drugs.

Description

technical field [0001] The invention relates to a method for making an animal model of simulating human pelvic inflammatory disease. Background technique [0002] Pelvic inflammatory disease refers to the inflammation of female internal reproductive organs and surrounding connective tissue and pelvic peritoneum. It includes endometritis, uterine myositis, salpingitis, ovarian inflammation, etc. Pelvic inflammatory disease is a common and refractory disease in gynecology. Severe cases can cause sepsis and sepsis. If not controlled in time, septic shock and even death may occur. It may also lead to menstrual disorders and even infertility. Its incidence is increasing. Clinical studies have shown that the common pathogenic factors of pelvic inflammatory disease are bacteria (including aerobic bacteria and anaerobic bacteria), as well as viruses, molds, mycoplasma, chlamydia, etc. At present, the animal model of pelvic inflammatory disease does not match the mechanism of h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 刘敏李玉桑唐和斌杨祥良
Owner 唐和斌
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