35results about How to "No special technical requirements" patented technology

The invention relates to a preparation method of animal model simulating human pelvic inflammatory disease (PID). The method comprises the steps of selecting animal of family Muridae without reproductive tract diseases; and under sterile condition, inoculating strain of genus Enterobacter, genus Staphylococcus or genus Candida in animal vagina for 1-8 weeks and 1-7 times every week. The invention has the advantages of high model preparation success ratio and no dependence on immunity inhibitor. After success of model preparation, vaginal secretion obviously increases; bacteria can be observed by vaginal smear microscopic examination; metryperemia, abscess and ovarian cyst can be observed after dissection. Large amount of inflammatory cell infiltration can be observed in uterus and oviduct by organ tissue section microscopic examination. Blood examination indicates abnormal rise of neutrophil count. The method has the advantages of simple implementation, easily-accessible materials, easy grasping without special technical requirements for operator, accordance with clinical natural occurrence process of PID, and suitability for research of generation, development and mechanism of PID and screening of relevant drugs.

The invention discloses a dissipative element of a grouting sleeve, comprising an inner tube and an outer tube which are arranged coaxially. The slit between the lap sections of the outer tube and the inner tube is provided with expanding cement slurry, and an expanding cement sheath is formed after the expanding cement slurry is cured. The pretension force of the expanding cement slurry enables the friction force of the contact surface between the expanding cement sheath and the sleeve body to be increased. At normal operating condition, the dissipative element can be used as ordinary support to bear axial loading; with the effect of earthquake loading, the dissipative element consumes and absorbs the earthquake energy by relative sliding of inner and outer tubes. The member has no too high requirement for machining precision, thereby saving steel material and having low cost. After the dissipative element is damaged and inactive in earthquake hazard, the dissipative element of a grouting sleeve is just needed to be replaced and the operation is very convenient.

The invention relates to a device for making a hydraulic model, and belongs to the technical field of hydraulic engineering. The device has an international patent classification (IPC) number of E02B 1 / 02, is suitable for making a hydraulic model with a steep side slope or a large terrain height difference, and consists of a beam, an elevation control system, a bracket and a river cross section form control line. When a hydraulic engineering physical model is made, a coordinate origin of a coordinate system is determined above a river cross section; the beam is taken as a horizontal baseline,and scales processed along the horizontal direction can control the starting distance of the river cross section; each elevation control rod in the elevation control system can move up and down, and left and right, and the model relative elevation of each elevation control point is accurately controlled through scales along the vertical direction; the river cross section form control line directly passes through small holes at the tip of the elevation control rods in turn to form a complete river cross section; and construction personnel can make the channel cross section accordingly and thenmake the whole physical model.

The invention relates to a manufacturing method of a hydraulic model, belonging to the technical field of hydraulic engineering. The IPC (International Patent Classification) of the hydraulic model is E02B 1 / 02. The manufacturing method is suitable for manufacturing the hydraulic model with a steep side slope or higher topographic height difference and adopts a special topographic manufacturing instrument comprising four parts, i.e. a cross beam, an elevation control system, a support frame and a riverway section morphologic line. When the hydraulic model is manufactured, the manufacturing method comprises the following steps of: firstly determining a coordinate origin of a coordinate system above a riverway section; controlling the starting point distance of the riverway section along a scale processed in a horizontal direction by taking the cross beam as a horizontal datum line; accurately controlling the relative model elevation of each elevation control point along a vertical scale in such a way that each elevation control rod of the elevation control system can move up and down or left and right; and enabling the riverway section morphologic line to directly sequentially penetrate through a small hole positioned on the top end of each elevation control rod so as to form a complete riverway section. Thus constructors can manufacture the riverway section so as to manufacture an integral entity model.

The invention provides an electrochemical method used for measuring activity of alkaline phosphatase and an electrochemical sensor used in the method. The method comprises the following steps: preparing an electrode modified by cysteine and copper ions; drawing a standard curve between the enzyme activity concentration of alkaline phosphatase and relative current intensity under a pyrophosphate ion substrate with specific concentration; changing the alkaline phosphatase solution with known enzyme activity concentration into a to-be-measured sample containing alkaline phosphatase, performing cyclic voltammetry scanning to obtain the relative current intensity, and calculating to obtain the activity concentration of the alkaline phosphatase in the to-be-measured sample according to the standard curve. According to the method disclosed by the invention, a reversible competitive coordination principle of the cysteine, pyrophosphate ions and copper ions is utilized, the electrochemical sensor used for measuring the activity of alkaline phosphatase is prepared, and the activity of alkaline phosphatase can be rapidly, simply and accurately measured by utilizing the electrochemical sensor.

The invention discloses a preparation method of Schiff base and an application thereof in detecting trace chromium in environmental wastewater. The chromium in the environmental wastewater is reduced to Cr(III) which reacts with Schiff base to form a complex; the Cr(III)-Schiff base complex in the sample is separated and enriched by CPE (cloud point extraction) using a nonionic surfactant / salt to form a yellow complex; and the trace chromium in the sample is detected by comparing the enrichment phase enriched on the surfactant with the standard chromium content, wherein the detection limit is 0.1mug / mL. The method disclosed by the invention has the advantages of simplicity in operation, high detection sensitivity, no use of organic solvent, environmental protection, short detection time and the like, is a simple, quick and accurate analysis method and has wide application prospect.

The invention discloses a method for measuring the amount of residual penicillin in milk. Standard working anhydrous alcohol solution with penicillin sodium is prepared, the pH value is regulated by dilute HCl to 2 plus or minus 0.5 or 4 plus or minus 0.5, the standard working anhydrous alcohol solution is bathed in boiling water, so that degradation products, i.e. penicillamine and penicillenic acid, are obtained, then the two degradation products respectively react with benzoquinone substance, so that charge-transfer complexes are obtained, and the working curves of different charge-transfer complexes are calculated. During measurement, the absorbance of the charge-transfer complex of milk sample is calculated by the same way, and is substituted into the working curve of the charge-transfer complex to work out the amount of the residual penicillin in the milk. The invention takes the lead in directly using the ultraviolet or fluorescent spectrophotometry to measure the amount of the residual penicillin in the milk. The method has the advantages of quick measurement, wide measurement range, high sensitivity, high accuracy and good selectivity, an expensive antibiotic detection instrument does not need to be purchased, the operation method is simple, and the method can reduce the production cost and the operation risk for diary processing enterprises, and therefore having a good application prospect.

The invention discloses a grouting sleeve dissipative element with a protection piece. The dissipative element consists of an inner tube and an outer tube which are coaxially lapped; external wall of the inner tube is provided with the protection piece consisting of metal skin, distributed steel bars and steel bar heads; a cavity is formed between the inner tube and the outer tube, and an expanding cement mantle is formed in the cavity. Contact surface between the expanding cement mantle and a steel tube are pressed tightly by chemical prestress of expanding cement grout to increase friction force, and the protection piece can protect internal surface of the expanding cement mantle and prevent reduction of bearing capacity due to cracking under reciprocating friction. The dissipative element can be taken as a common support to bear axial load in a normal service status, and the dissipative element consumes and absorbs seismic energy by relative slip of the inner tube and the outer tube under the action of seismic load. The dissipative element has the advantages of low requirement for machining accuracy, steel saving and low cost. Only the grouting sleeve dissipative element needs to be replaced if the grouting sleeve dissipative element is damaged and disabled in a seismic hazard, which is very convenient.

The invention relates to an energy dissipating element of a grouting sleeve, which belongs to the field of structural engineering for fixing buildings and comprises an inner tube and an outer tube. A cover plate is arranged at one end of the inner tube, the inner tube together with the cover plate is divided into a plurality of independent single blocks along the axis direction of the inner tube, each single block consists of a tubular wall and a cover plate block, the outer wall of the inner tube which is divided into blocks is attached onto the inner wall of the outer tube and inserted into the outer tube so that an inner tube insertion section and an inner tube exposed section are formed, the cover plate which is divided into blocks is disposed in the end of the inner tube insertion section, and expanding cement grout is injected into the inner tube insertion section through the inner tube exposed section, so that an expanding cement grout body is formed. The energy dissipating element of the grouting sleeve is capable of guaranteeing construction quality and increasing friction among sleeves effectively, has the advantages of convenience in processing and mounting, economy and reliability, and can be used as a structural load-bearing component when in an earthquake non-resistant state.

The invention relates to a fluorescence detection method for the activity of alkaline phosphatase, which is characterized in that sodium 4-hydroxyphenyl phosphate (4-HPP) is used as a substrate, molecules of the substrate are hydrolyzed into 1,4-benzenediol (HQ) under the catalysis of alkaline phosphatase in an aqueous solution, 1,4-benzenediol can react with subsequently added 3-(2-aminoethylamino)propyltrimethoxysilane (DAMO) to generate silicon nanoparticles (SiNPs) capable of emitting green fluorescence. The higher the activity of alkaline phosphatase in the system is, the more the generated fluorescent silicon nanoparticles are, and the higher the fluorescence intensity is. Quantitative analysis and detection of the activity of alkaline phosphatase are carried out according to the fluorescence intensity change of the detection solution. Compared with the prior art, the method has the advantages that required reagents and medicines are simple and easy to obtain, the operation is convenient, quick and accurate, and a sensitive and reliable method is provided for quantitative detection of the activity of alkaline phosphatase in a clinical sample. According to the fluorescence detection method, the detection limit is 0.1mU / mL, and the fluorescence detection method has the advantages of high sensitivity, high selectivity, wide linear range and the like.

The invention relates to the technical field of agricultural facilities and discloses a small greenhouse fast to insert and install. The small greenhouse comprises a roof frame, stand columns and a base. The base comprises a plurality of straight sleeves, three-way sleeves and house corner sleeves. Three ports of each house corner sleeve are perpendicular to one another. The straight sleeves are connected in series through the three-way sleeves to form the side edges of the base. The adjacent side edges are connected through the house corner sleeves to form a quadrangle frame of the base. The lengths of the opposite side edges of the quadrangle frame of the base are equal. The stand columns are inserted and connected to the upper ports of the three-way sleeves and the upper ports of the house corner sleeves. A roof frame cover is arranged at the top ends of the stand columns. Coverage grids are installed on the outer surface of the roof frame and between the adjacent stand columns. The temporary greenhouse can be set up fast at low cost, the greenhouse is flexible to use due to the size and the height, the installing mode is simple, and the needs for material storage space are small.

The invention discloses a reinforced grouting sleeve dissipative element. The dissipative element consists of an inner tube and an outer tube which are coaxially lapped; a cavity is formed between the inner tube and the outer tube, a cement mantle is formed in the cavity, and stirrups are arranged in a grouting cavity. Friction force of contact surface between the expanding cement mantle and the tube is increased by chemical prestress of expanding cement grout; the stirrups can restrain expansion of cracks and prevent the expanding cement grout from cracking so as to improve bearing capacity and hysteretic energy dissipation properties of the dissipative element. The dissipative element can be taken as a common support to bear axial load in a normal service status, and the dissipative element consumes and absorbs seismic energy by relative slip of the inner tube and the outer tube under the action of seismic load. The dissipative element has the advantages of low requirement for machining accuracy, steel saving and low cost. Only the grouting sleeve dissipative element needs to be replaced if the grouting sleeve dissipative element is damaged and disabled in a seismic hazard, which is very convenient.

The invention provides a method for detecting β-lactam antibiotic residues in milk. The residues of β-lactam antibiotics in milk are detected by three steps of centrifugation, chemical reaction of β-lactam antibiotics and heavy metals, and UV spectrophotometry. The damage is small; the detection speed is fast, and the entire operation process can be completed within 30 minutes; no need to purchase expensive antibiotic detection instruments, only a low-speed centrifuge and a UV spectrophotometer can be used to complete the detection.