Method for extracting high-purity caffeine from tea leaves

A caffeine and high-purity technology, applied in organic chemistry and other directions, can solve the problems of loss of caffeine and low yield, and achieve the effects of cost saving, high efficiency and small loss rate

Active Publication Date: 2016-03-16
YUNNAN CHANONG BIOLOGICAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The caffeine extracted by this method is not only yellow, but also loses too much caffeine during the preparation process, and the yield is low; (2) Extracting caffeine from tea leaves by direct sublimation without solvent: the caffeine extracted by this method has a slight Yellow, this is caused by the tar produced by the burning of some materials during the sublimation process, which needs further purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting high-purity caffeine from tea leaves, comprising the following steps:

[0037] Step (1), extraction: freeze and pulverize the fresh tea leaves at minus 20 to a particle size of 100 mesh, then add to ethanol whose volume is 3 times the volume of fresh tea leaves, perform ultrasonic extraction for 8 minutes, centrifuge, and discard the precipitate to obtain Extraction solution;

[0038] Step (2), separation: filter the extract obtained in step (1) with an ultrafiltration membrane with a cut-off flow rate of 30kD-60kD, take the filtrate, concentrate it with MVR, and concentrate it until the weight is 1 / 7 of the weight of the filtrate, then use XAD7 large Pore ​​adsorption resin, first use the first eluent to elute, remove caffeine, and then use the second eluent to elute to obtain the eluent containing tea polyphenols; Concentrate the eluent containing tea polyphenols to No alcohol smell, dry to get tea polyphenols; Concentrate the caffeine-contain...

Embodiment 2

[0045] A method for extracting high-purity caffeine from tea leaves, comprising the following steps:

[0046]Step (1), extraction: freeze and pulverize the fresh tea leaves at minus 25°C until the particle size is 100 mesh, then add to ethanol with a volume 5 times the volume of the fresh tea leaves, ultrasonically extract for 10 minutes, centrifuge, discard the precipitate, get the extract;

[0047] Step (2), separation: filter the extract obtained in step (1) with an ultrafiltration membrane with a cut-off flow rate of 30kD-60kD, take the filtrate, concentrate it with MVR, and concentrate it until the weight is 1 / 9 of the weight of the filtrate, then use XAD7 large Pore ​​adsorption resin, first use the first eluent to elute, remove caffeine, and then use the second eluent to elute to obtain the eluent containing tea polyphenols; Concentrate the eluent containing tea polyphenols to No alcohol smell, dry to get tea polyphenols; Concentrate the caffeine-containing eluent to n...

Embodiment 3

[0054] A method for extracting high-purity caffeine from tea leaves, comprising the following steps:

[0055] Add it to ethanol whose volume is 4 times the volume of the tea leaves, perform ultrasonic extraction for 9 minutes, centrifuge, discard the precipitate, and obtain the extract;

[0056] Step (2), separation: filter the extract obtained in step (1) with an ultrafiltration membrane with a cut-off flow rate of 30kD-60kD, take the filtrate, concentrate it with MVR until the weight is 1 / 8 of the weight of the filtrate, and use XAD7 large Pore ​​adsorption resin, first use the first eluent to elute, remove caffeine, and then use the second eluent to elute to obtain the eluent containing tea polyphenols; Concentrate the eluent containing tea polyphenols to No alcohol smell, dry to get tea polyphenols; Concentrate the caffeine-containing eluent to no alcohol smell, extract with ethyl acetate, take the organic phase, remove the solvent by rotary evaporation, and obtain the cru...

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PUM

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Abstract

The invention relates to a method for extracting high-purity caffeine from tea leaves and belongs to the technical field of extraction. By-product caffeine crude products obtained during production of tea polyphenols are utilized, caffeine is crystallized in water, the method is safe and free of toxicity, cost is reduced, and both the value and the income are increased. The method is easy to operate and environmentally friendly, the energy consumption of the film separation technology is low, selectivity is high, and a device is flexible; a column chromatography method is simple in operation technology, high in efficiency and good in safety; due to the method that the film separation technology is combined with column chromatography, tea polysaccharide, tea polyphenols, theanine and other components are effectively separated, and subsequent crystallization and purification of caffeine are facilitated; re-crystallization is performed through water, and the method is safe, free of toxicity, environmentally friendly, high in yield and low in cost; the method has the advantages that energy consumption is low in the freezing and crystallizing process, no solvents residuals exist, and product purity is high, the method is simple, both the value and the income are increased, and the method has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of extraction, and in particular relates to a method for extracting high-purity caffeine from tea leaves. Background technique [0002] Caffeine is a class of functional substances with health benefits, and has been widely used in industries such as industry, agriculture and medicine. The content of caffeine in tea is relatively high. At present, the methods for extracting and preparing caffeine from tea mainly include (1) traditional extraction methods: organic solvent extraction, sublimation, and precipitation; (2) new extraction technologies: column chromatography, Microwave extraction technology, supercritical extraction method, membrane separation method. [0003] The traditional crystallization methods of caffeine in tea are as follows: (1) Taking advantage of the properties that caffeine in tea is easily soluble in 80°C hot water, polyphenols in tea can form insoluble complexes with calcium ions, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D473/12
CPCC07D473/12
Inventor 李定忠
Owner YUNNAN CHANONG BIOLOGICAL IND
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